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kevinblighe / EnhancedVolcano

Publication-ready volcano plots with enhanced colouring and labeling
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why does the plot show mirrored datapoints in left and right side of the volcano plot? #103

Closed levinhein closed 2 years ago

levinhein commented 2 years ago

Hello. Could you help me please? I'm using EnhancedVolano in Seurat scRNA-seq analysis. I used FindAllMarkers() to analyze the DEGs between the 2 groups inside my seurat object.

I was expecting to see a volcanoplot where the data points on the left and right are not mirrors of each other like in your vignette (https://bioconductor.org/packages/release/bioc/vignettes/EnhancedVolcano/inst/doc/EnhancedVolcano.html). But it is mirrored when I use it. Could you help me resolve this challenge please?

*** FindAllMarkers() image

*** Data structure of the result of FindAllMarkers() image image

***EnhancedVolcano Visualization

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What should I do to correct this?

levinhein commented 2 years ago

Oh, I just discovered that the reason for this is because I used FindAllMarkers().

Just now, I used FindMarkers() instead, and the output is no longer mirrored.

Thank you!

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