Closed levinhein closed 2 years ago
Hello. Could you help me please? I'm using EnhancedVolano in Seurat scRNA-seq analysis. I used FindAllMarkers() to analyze the DEGs between the 2 groups inside my seurat object.
I was expecting to see a volcanoplot where the data points on the left and right are not mirrors of each other like in your vignette (https://bioconductor.org/packages/release/bioc/vignettes/EnhancedVolcano/inst/doc/EnhancedVolcano.html). But it is mirrored when I use it. Could you help me resolve this challenge please?
*** FindAllMarkers()
*** Data structure of the result of FindAllMarkers()
***EnhancedVolcano Visualization
What should I do to correct this?
Oh, I just discovered that the reason for this is because I used FindAllMarkers().
Just now, I used FindMarkers() instead, and the output is no longer mirrored.
Thank you!
Hello. Could you help me please? I'm using EnhancedVolano in Seurat scRNA-seq analysis. I used FindAllMarkers() to analyze the DEGs between the 2 groups inside my seurat object.
I was expecting to see a volcanoplot where the data points on the left and right are not mirrors of each other like in your vignette (https://bioconductor.org/packages/release/bioc/vignettes/EnhancedVolcano/inst/doc/EnhancedVolcano.html). But it is mirrored when I use it. Could you help me resolve this challenge please?
*** FindAllMarkers()![image](https://user-images.githubusercontent.com/68268222/163540626-f4549adc-acdc-4b64-9c65-b6329e2b40a0.png)
*** Data structure of the result of FindAllMarkers()
![image](https://user-images.githubusercontent.com/68268222/163540996-61abc468-5083-4c1b-9239-45f1b14543c0.png)
***EnhancedVolcano Visualization
What should I do to correct this?