linnabrown / run_dbcan

Run_dbcan V4, using genomes/metagenomes/proteomes of any assembled organisms (prokaryotes, fungi, plants, animals, viruses) to search for CAZymes.
http://bcb.unl.edu/dbCAN2
GNU General Public License v3.0
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cgc predict fail #177

Open Panda-smile opened 1 month ago

Panda-smile commented 1 month ago

@Report

!/bin/bash

SBATCH -J NAMD

SBATCH -p batch

SBATCH -N 1

SBATCH --ntasks-per-node=32

source ~/miniconda3/etc/profile.d/conda.sh conda activate /public/home/lj/miniconda3/envs/dbcan

run_dbcan /public/home/lj/CazyDB/temp/qc/Saccharomycopsisproteins.faa protein --dbcan_thread 32 --tf_cpu 32 --stp_cpu 32 -c /public/home/lj/CazyDB/temp/qc/Saccharomycopsis.gff --cgc_substrate --hmm_cpu 32 --out_dir SF.PUL --dia_cpu 32 --db_dir /public/home/lj/CazyDB/db

Hello Professor,

I would like to seek your guidance on an issue. After running the aforementioned code, I did not obtain the expected predictions related to cgc predict results (such as PUL and synpdf). I am wondering if this issue might be related to the input file I used. I have attached my gff format file, which is the result of an output from the funnannotate software. Could you please advise me on how to modify or process my GFF file to meet the requirements of dbCAN?I am looking forward to your reply.

Best regards Panda-smile image

image

1)gff文件(已做后缀修改,替换为txt):Saccharomycopsis.txt

2)蛋白序列为:Saccharomycopsisproteinsfaa.txt

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