I have encountered an issue whereby SSW gives different results depending on the association of sequences as a read or as a reference. Ie. when one of the sequences is a read and the other is a reference, I get a proper alignment. When their association is reversed, I get no alignment. I've written a small C program that can replicate this behavior, and I've tested this with multiple versions of SSW only to get the same result. Example program, compile command, and subsequent output are below. You can download bug.c from here.
I have encountered an issue whereby SSW gives different results depending on the association of sequences as a read or as a reference. Ie. when one of the sequences is a read and the other is a reference, I get a proper alignment. When their association is reversed, I get no alignment. I've written a small C program that can replicate this behavior, and I've tested this with multiple versions of SSW only to get the same result. Example program, compile command, and subsequent output are below. You can download bug.c from here.
int main (int argc, char * const argv[]) { int32_t gap_open = 3, gap_extension = 1;
}
Thanks!