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miaozhun / DEsingle

Homepage: https://miaozhun.github.io/DEsingle. Bioconductor page: https://bioconductor.org/packages/DEsingle.
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Add parameter to skip Normalization step? #9

Open mr-september opened 3 years ago

mr-september commented 3 years ago

I have pre-normalized the data in Seurat, then converted to sce to run DEsingle (as discussed in Seurat's issues, counts=logcounts), but it seems to be re-normalizing the normalized data.

Can an argument be added to DEsingle command to not do further normalization?

miaozhun commented 3 years ago

I suggest to use DEsingle's normalization step with your data or re-normalize the normalized data, since DEsingle's normalization step could ensure the data work with ZINB distribution.