##### GENERATING VCF OUTPUT...Reference sequence used for VCF: RSRS
##### PREDICTING HAPLOGROUPS AND ANNOTATING/PRIORITIZING VARIANTS...
Haplogroup predictions based on RSRS Phylotree build 17Unable to compute haplogroup. ExitYour best results file is mt_classification_best_results.csv
Loading contig sequences from file PM18_case-contigs.fastaUnable to compute haplogroup. ExitYour best results file is mt_classification_best_results.csv
Loading contig sequences from file PM18_ctrl-contigs.fastaParsing pathogenicity table...Parsing variability data...Parsing info about haplogroup-defining sites...Parsing info about haplogroup assignments...No annotation.csv found. Exit
If I run MToolBox leaving the default configuration, it works.
I tried to run MToolBox with the following config file:
mtdb_fasta=chrRSRS.fa
hg19_fasta=hg19RSRS.fa
mtdb=RSRS
humandb=hg19RSRS
input_type=bam
ref=RSRS
But the last steps of the run failed:
##### GENERATING VCF OUTPUT...
Reference sequence used for VCF: RSRS
##### PREDICTING HAPLOGROUPS AND ANNOTATING/PRIORITIZING VARIANTS...
Haplogroup predictions based on RSRS Phylotree build 17
Unable to compute haplogroup. ExitYour best results file is mt_classification_best_results.csv
Loading contig sequences from file PM18_case-contigs.fasta
Unable to compute haplogroup. ExitYour best results file is mt_classification_best_results.csv
Loading contig sequences from file PM18_ctrl-contigs.fasta
Parsing pathogenicity table...
Parsing variability data...
Parsing info about haplogroup-defining sites...
Parsing info about haplogroup assignments...
No annotation.csv found. Exit
If I run MToolBox leaving the default configuration, it works.
input_type=bam
ref=RSRS
I've performed the default MToolBox installation.