error occured whe running Epinano_Variants.py

novoalab / EpiNano

Detection of RNA modifications from Oxford Nanopore direct RNA sequencing reads (Liu*, Begik* et al., Nature Comm 2019)

GNU General Public License v2.0

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error occured whe running Epinano_Variants.py #149

Open Seongmin-Jang-1165 opened 1 month ago

Seongmin-Jang-1165 commented 1 month ago

hello

i ran Epinano for analyze m6a modification with oure Direct RNA seq data

but when i started, it did not work and the error message is below:

Please offer correctly path to sam2tsv.jar

how can i fix this error?

i ran Epinano under Bioconda Environment

enovoa commented 1 month ago

Please check the version of Epinano you are using, as stated in the README and the description of Epinano_variants.py, Epinano v1.2.4 does not rely on sam2tsv.jar any more

Seongmin-Jang-1165 commented 1 month ago

@enovoa thanks for advice!

does basecalled data for Epinano must be generated using Guppy3.1.5? my data is POD5 format and i tried to run Epinano with DORADO basecaller