chip

[qPCR4vir]

32 Probe for:

• [x] PanFlav
• [ ] Alpha
• [ ] Buny
• [ ] NairoV

Left 20 (13)

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"Pan PCR" for:

• [X] PanFlav
• [X] Alpha
• [ ] Buny
• [ ] NairoV

[qPCR4vir]

PanFlav

PFlav-fAAR_326 PanFlav TACAACATGATGGGAAAGAGAGAGAARAA

PFlav-rKR_327 PanFlav
GTGTCCCAKCCRGCTGTGTCATC

[qPCR4vir]

Pan Alpha, Vina-Rodriguez, 2017. Modif Eshoo.

A_VIR966_f Vina-Rodriguez et al. 2015. Modif Eshoo. PanAlpha (58-62°C) TCCATGCTAATGCYAGAGCGTTTTCGCA

A_VIR966.R Vina-Rodriguez et al. 2015. Modif Eshoo. PanAlpha (28-49°C) TGGCGCACTTCCAATGTCHAGGAT

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V_p Vina-Rodriguez et al. 2015. Modif Eshoo. VEEV (25-63°C) TGATCGARACGGAGGTRGAMCCATCC

[qPCR4vir]

Cal_Bwa_group_forward Orthobunyavirus N ORF, 210nt . Lambert-Lanciotti-2009, Bunya-S
GCAAATGGATTTGATCCTGATGCAG

Cal_Bwa_group_reverse Orthobunyavirus N ORF, 210nt. Lambert-Lanciotti-2009, Bunya-S
TTGTTCCTGTTTGCTGGAAAATGAT

Bun_group_forward Orthobunyavirus N ORF, 250nt. Lambert-Lanciotti-2009, Bunya-S
CTGCTAACACCAGCAGTACTTTTGAC

Bun_group_reverse Orthobunyavirus N ORF, 250nt. Lambert-Lanciotti-2009, Bunya-S
TGGAGGGTAAGACCATCGTCAGGAACTG

Wyeomyia_forward Orthobunyavirus N ORF, 230nt. Lambert-Lanciotti-2009, Bunya-S
ATGTCTGAAATTGTATTTGATGATATTGG

Wyeomyia_reverse Orthobunyavirus N ORF, 230nt. Lambert-Lanciotti-2009, Bunya-S
TATTTCGATTCCCCGGAAAGT

Oropouche_forward Orthobunyavirus N ORF, 300nt. Lambert-Lanciotti-2009, Bunya-S
GGCCCATGGTTGACCTTACTTT

Oropouche_reverse Orthobunyavirus N ORF, 300nt. Lambert-Lanciotti-2009, Bunya-S
ACCAAAGGGAAGAAAGTGAAT

Phlebo_forward_1 Phlebovirus N ORF, 370 nt. Lambert-Lanciotti-2009, Bunya-S
TTTGCTTATCAAGGATTTGATGC

Phlebo_reverse Phlebovirus N ORF, 370 nt. Lambert-Lanciotti-2009, Bunya-S
TCAATCAGTCCAGCAAAGCTGGGATGCATCAT

Phlebo_forward_2 Phlebovirus N ORF, 370 nt. Lambert-Lanciotti-2009, Bunya-S
TTTGCTTATCAAGGATTTGACC

[qPCR4vir]

Pan NairoV, Lambert-Lanciotti-2009, Bunya-S ?

Nairo_forward #530. Nairovirus S - N ORF, 400 nt. Lambert-Lanciotti-2009, Bunya-S
TCTCAAAGAAACACGTGCCGC

Nairo_reverse #527. Nairovirus S - N ORF, 400 nt. Lambert-Lanciotti-2009, Bunya-S
GTCCTTCCTCCACTTGWGRGCAGCCTGCTGGTA

CCHFV-S, Sas, Vina-Rodriguez-2018

Sas, Vina-Rodriguez-2018

CCHF-I-f Genome positiona 1,068–1,095. Sas, Vina-Rodriguez-2018 CAAGAGGCACTAAAAAAATGAAGAAGGC

CCHF-II-f Genome positiona 1,068–1,095. Sas, Vina-Rodriguez-2018 CAAGGGGYACCAARAAAATGAAGAAGGC

CCHF-III-f Genome positiona 1,068–1,095. Sas, Vina-Rodriguez-2018 CAAGAGGTACCAAGAAAATGAAGAAGGC

CCHF-IV-f Genome positiona 1,068–1,095. Sas, Vina-Rodriguez-2018 CAAGGGGTACCAAGAAAATGAAGAARGC

CCHF-V-f Genome positiona 1,068–1,095. Sas, Vina-Rodriguez-2018 CAAGGGGGACCAARAAAATGAAAAAGGC

CCHF-VI-f Genome positiona 1,068–1,095. Sas, Vina-Rodriguez-2018 CAAGGGGCACCAAGAAAATGAAGAAAGC

CCHF-deg-f Genome positiona 1,068–1,095. Sas, Vina-Rodriguez-2018 CAAGGGGKACCAAGAAAATGAARAAGGC

CCHF-I-r 1,223–1,248. Sas, Vina-Rodriguez-2018 GCAACAGGGATGGTTCCAAAGCAAAC

CCHF-II-r 1,223–1,248. Sas, Vina-Rodriguez-2018 GCYACRGGGATGGTTCCRAAGCAGAC

CCHF-III-r 1,223–1,248. Sas, Vina-Rodriguez-2018 GCCACGGGGATTGTCCCAAAGCAGAC

CCHF-IV-r 1,223–1,248. Sas, Vina-Rodriguez-2018 GCCACAGGGATTGTTCCAAAGCAGAC

CCHF-V-r 1,223–1,248. Sas, Vina-Rodriguez-2018 GCAACAGGGATTGTTCCAAAGCAGAC

CCHF-VI-r 1,223–1,248. Sas, Vina-Rodriguez-2018 GCTACAGGAATTGTCCCAAAGCAGAC

CCHF-deg-r 1,223–1,248. Sas, Vina-Rodriguez-2018 GCMACAGGGATTGTYCCAAAGCAGAC

CCHF-probe-1 6-FAM--BHQ1 1,172–1,198. Sas, Vina-Rodriguez-2018 ATCTACATGCACCCTGCYGTGYTGACA

CCHF-probe-2 6-FAM- -BHQ1 1,101–1,128. Sas, Vina-Rodriguez-2018 TTCTTCCCCCACTTCATTGGRGTGCTCA

uArr CCHFV-S Wolfel et al. 2009. pos U88410:210-242 ?