qqwang-berkeley / JUM

A tool for annotation-free differential analysis of tissue-specific pre-mRNA alternative splicing patterns
MIT License
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Error running R_script_JUM.R #18

Closed kjsawicka closed 5 years ago

kjsawicka commented 5 years ago

I am trying to run through the JUM pipeline comparing aligned sequencing data from 2 conditions each with 4 replicates.

I got as far as running R_script_JUM.R but then I encountered a problem. The error message was:

Error in .Call2("solve_user_SEW0", start, end, width, PACKAGE = "IRanges") : solving row 3717: range cannot be determined from the supplied arguments (too many NAs)

Looking into it a bit further it seems the error is caused by some rows in the combined_AS_JUM.gff file not having both start and end genomic coordinates. Do you have any suggestions as I can't work out where the issue is coming from? Thanks.