I'm querying my .gff files to pull out sequences for certain genes but it is giving me output in amino acids. I saw in a supplementary document that you can get this outputted in nucleotides but the documentation does not say how. Does anyone know how to do this?
Also for some of my genes, I am not getting all the sequences outputted. If a gene sequence is 100% identical across all isolates does it only output one sequence?
renrok
commented
2 months ago
I'm querying my .gff files to pull out sequences for certain genes but it is giving me output in amino acids. I saw in a supplementary document that you can get this outputted in nucleotides but the documentation does not say how. Does anyone know how to do this?
Also for some of my genes, I am not getting all the sequences outputted. If a gene sequence is 100% identical across all isolates does it only output one sequence?