As zUMIs prefers fastq files that are not demultiplexed, I ran merge_demutliplexed_fastq.R.
It generated 3 fastq files (R1, R2 and index reads), which seems ok.
The file that I'm concerned is reads_for_zUMIs.expected_barcodes.txt.
As I noticed, the barcodes are random characters [A-Z] of length of 8,
Which are generated by stri_rand_strings function in stringi package.
I'm wondering the use of these random characters. Can they be used as input for expected barcodes in zUMI config?
*P.S
I'm dealing with Smart Seq3 libraries so I know the barcodes used in my experiment. Should I use these as input for expected barcodes in the config?
Hi, thanks for providing wonderful tool !
As zUMIs prefers fastq files that are not demultiplexed, I ran merge_demutliplexed_fastq.R.
It generated 3 fastq files (R1, R2 and index reads), which seems ok.
The file that I'm concerned is
reads_for_zUMIs.expected_barcodes.txt
.As I noticed, the barcodes are random characters [A-Z] of length of 8,
Which are generated by
stri_rand_strings
function in stringi package.I'm wondering the use of these random characters. Can they be used as input for expected barcodes in zUMI config?
*P.S I'm dealing with Smart Seq3 libraries so I know the barcodes used in my experiment. Should I use these as input for expected barcodes in the config?
Again, thank you for your support !