uclahs-cds / pipeline-call-sSNV

A Nextflow pipeline to identify the somatic single nucleotide variants (sSNVs) by comparing a pair of tumor/normal samples.
https://uclahs-cds.github.io/pipeline-call-sSNV/
GNU General Public License v2.0
5 stars 0 forks source link

update resource allocations #289

Closed sorelfitzgibbon closed 6 months ago

sorelfitzgibbon commented 6 months ago

Description

Resource allocations updated based on results of 30 PCAWG and 13 SARC (SGSALASC) sarcoma sample runs. Main changes for F72:

PCAWG samples described here SARC samples described here

Sample coverage

dataset type min max average
PCAWG tumor 24x 71x 44x
PCAWG normal 24x 50x 32x
SARC tumor 18x 96x 75x
SARC normal 36x 89x 62.5x

Closes #255 #283

Testing Results

Tested on different PCAWG-63 samples that had failed before this update. They all failed again initially and worked on retry.

main logs:

/hot/code/sfitzgibbon/gitHub/uclahs-cds/pipeline-call-sSNV/sfitz-update-resources_DO220599_ssnv.log /hot/code/sfitzgibbon/gitHub/uclahs-cds/pipeline-call-sSNV/sfitz-update-resources_DO36881_ssnv.log /hot/code/sfitzgibbon/gitHub/uclahs-cds/pipeline-call-sSNV/sfitz-update-resources_DO37758_ssnv.log /hot/code/sfitzgibbon/gitHub/uclahs-cds/pipeline-call-sSNV/sfitz-update-resources_DO38847_ssnv.log

process logs:

/hot/user/sfitzgibbon/PCAWG-63/data/pipeline-runs/sfitz-update-resources-DO220599-ssnv/call-sSNV-8.0.0/SA556937/log-call-sSNV-8.0.0-20240507T214647Z /hot/user/sfitzgibbon/PCAWG-63/data/pipeline-runs/sfitz-update-resources-DO36881-ssnv/call-sSNV-8.0.0/SA421337/log-call-sSNV-8.0.0-20240507T222604Z /hot/user/sfitzgibbon/PCAWG-63/data/pipeline-runs/sfitz-update-resources-DO37758-ssnv/call-sSNV-8.0.0/SA433958/log-call-sSNV-8.0.0-20240508T172838Z /hot/user/sfitzgibbon/PCAWG-63/data/pipeline-runs/sfitz-update-resources-DO38847-ssnv/call-sSNV-8.0.0/SA443714/log-call-sSNV-8.0.0-20240508T181628Z

Testing after updating submodules, nftest

main log: /hot/software/pipeline/pipeline-call-sSNV/Nextflow/development/unreleased/sfitz-update-resources/slurm-154972.out process log: /hot/software/pipeline/pipeline-call-sSNV/Nextflow/development/unreleased/sfitz-update-resources/log-nftest-20240513T232120Z.log

Checklist

github-actions[bot] commented 6 months ago

Bleep bloop, I am a robot.

Alas, some of the Nextflow configuration tests failed!

test/configtest-F16.json

@ ["params","proc_resource_params","call_sIndel_Manta","retry_strategy","memory","operand"]
- "3 GB"
+ "5 GB"
@ ["params","proc_resource_params","call_sSNV_Mutect2","memory"]
- "3 GB"
+ "2 GB"
@ ["params","proc_resource_params","convert_BAM2Pileup_SAMtools","retry_strategy","memory","operand"]
- "3 GB"
+ "5 GB"
@ ["params","proc_resource_params","create_IndelCandidate_SAMtools","retry_strategy","memory","operand"]
- "3 GB"
+ "5 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","operand"]
- "2"
+ "20 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","strategy"]
- "exponential"
+ "add"
@ ["params","proc_resource_params","run_sump_MuSE","memory"]
- "24 GB"
+ "16 GB"
@ ["params","proc_resource_params","run_sump_MuSE","retry_strategy","memory","operand"]
- "8 GB"
+ "16 GB"
@ ["process","withName:call_sIndel_Manta","memory","2"]
- "9 GB"
+ "11 GB"
@ ["process","withName:call_sIndel_Manta","memory","3"]
- "12 GB"
+ "16 GB"
@ ["process","withName:call_sIndel_Manta","memory","closure"]
- "retry_updater(6 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(6 GB, add, 5 GB, $task.attempt, memory)"
@ ["process","withName:call_sSNV_Mutect2","memory","1"]
- "3 GB"
+ "2 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","2"]
- "5 GB"
+ "4 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","3"]
- "7 GB"
+ "6 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","closure"]
- "retry_updater(3 GB, add, 2 GB, $task.attempt, memory)"
+ "retry_updater(2 GB, add, 2 GB, $task.attempt, memory)"
@ ["process","withName:convert_BAM2Pileup_SAMtools","memory","2"]
- "4 GB"
+ "6 GB"
@ ["process","withName:convert_BAM2Pileup_SAMtools","memory","3"]
- "7 GB"
+ "11 GB"
@ ["process","withName:convert_BAM2Pileup_SAMtools","memory","closure"]
- "retry_updater(1 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(1 GB, add, 5 GB, $task.attempt, memory)"
@ ["process","withName:create_IndelCandidate_SAMtools","memory","2"]
- "4 GB"
+ "6 GB"
@ ["process","withName:create_IndelCandidate_SAMtools","memory","3"]
- "7 GB"
+ "11 GB"
@ ["process","withName:create_IndelCandidate_SAMtools","memory","closure"]
- "retry_updater(1 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(1 GB, add, 5 GB, $task.attempt, memory)"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","2"]
- "16 GB"
+ "28 GB"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","closure"]
- "retry_updater(8 GB, exponential, 2, $task.attempt, memory)"
+ "retry_updater(8 GB, add, 20 GB, $task.attempt, memory)"
@ ["process","withName:run_sump_MuSE","memory","1"]
- "24 GB"
+ "16 GB"
@ ["process","withName:run_sump_MuSE","memory","closure"]
- "retry_updater(24 GB, add, 8 GB, $task.attempt, memory)"
+ "retry_updater(16 GB, add, 16 GB, $task.attempt, memory)"

test/configtest-F32.json

@ ["params","proc_resource_params","call_sSNV_Mutect2","memory"]
- "3 GB"
+ "2 GB"
@ ["params","proc_resource_params","call_sSNV_Mutect2","retry_strategy","memory","operand"]
- "3 GB"
+ "2 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","memory"]
- "16 GB"
+ "12 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","operand"]
- "2"
+ "20 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","strategy"]
- "exponential"
+ "add"
@ ["params","proc_resource_params","run_sump_MuSE","memory"]
- "48 GB"
+ "18 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","1"]
- "3 GB"
+ "2 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","2"]
- "6 GB"
+ "4 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","3"]
- "9 GB"
+ "6 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","closure"]
- "retry_updater(3 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(2 GB, add, 2 GB, $task.attempt, memory)"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","1"]
- "16 GB"
+ "12 GB"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","3"]
- "64 GB"
+ "52 GB"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","closure"]
- "retry_updater(16 GB, exponential, 2, $task.attempt, memory)"
+ "retry_updater(12 GB, add, 20 GB, $task.attempt, memory)"
@ ["process","withName:run_sump_MuSE","memory","1"]
- "48 GB"
+ "18 GB"
@ ["process","withName:run_sump_MuSE","memory","2"]
- "64 GB"
+ "34 GB"
@ ["process","withName:run_sump_MuSE","memory","3"]
- "64 GB"
+ "50 GB"
@ ["process","withName:run_sump_MuSE","memory","closure"]
- "retry_updater(48 GB, add, 16 GB, $task.attempt, memory)"
+ "retry_updater(18 GB, add, 16 GB, $task.attempt, memory)"

If the above changes are surprising, stop and determine what happened.

If the above changes are expected, there are two ways to fix this:

  1. Automatically: Post a comment starting with "/fix-tests" (without the quotes) and I will update the tests for you (you must review my work afterwards).
  2. Manually: Follow these steps on Confluence.
sorelfitzgibbon commented 6 months ago

/fix-tests

github-actions[bot] commented 6 months ago

Bleep bloop, I am a robot.

I have updated all of the failing tests for you with a3a311978a0ff60d88c963c05b06b1ab35358cda. You must review my work before merging this pull request!

github-actions[bot] commented 6 months ago

Bleep bloop, I am a robot.

Alas, some of the Nextflow configuration tests failed!

test/configtest-F16.json

@ ["params","proc_resource_params","call_sIndel_Manta","retry_strategy","memory","operand"]
- "3 GB"
+ "5 GB"
@ ["params","proc_resource_params","call_sSNV_Mutect2","memory"]
- "3 GB"
+ "2 GB"
@ ["params","proc_resource_params","convert_BAM2Pileup_SAMtools","retry_strategy","memory","operand"]
- "3 GB"
+ "5 GB"
@ ["params","proc_resource_params","create_IndelCandidate_SAMtools","retry_strategy","memory","operand"]
- "3 GB"
+ "5 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","operand"]
- "2"
+ "20 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","strategy"]
- "exponential"
+ "add"
@ ["params","proc_resource_params","run_sump_MuSE","memory"]
- "24 GB"
+ "16 GB"
@ ["params","proc_resource_params","run_sump_MuSE","retry_strategy","memory","operand"]
- "8 GB"
+ "16 GB"
@ ["process","withName:call_sIndel_Manta","memory","2"]
- "9 GB"
+ "11 GB"
@ ["process","withName:call_sIndel_Manta","memory","3"]
- "12 GB"
+ "16 GB"
@ ["process","withName:call_sIndel_Manta","memory","closure"]
- "retry_updater(6 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(6 GB, add, 5 GB, $task.attempt, memory)"
@ ["process","withName:call_sSNV_Mutect2","memory","1"]
- "3 GB"
+ "2 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","2"]
- "5 GB"
+ "4 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","3"]
- "7 GB"
+ "6 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","closure"]
- "retry_updater(3 GB, add, 2 GB, $task.attempt, memory)"
+ "retry_updater(2 GB, add, 2 GB, $task.attempt, memory)"
@ ["process","withName:convert_BAM2Pileup_SAMtools","memory","2"]
- "4 GB"
+ "6 GB"
@ ["process","withName:convert_BAM2Pileup_SAMtools","memory","3"]
- "7 GB"
+ "11 GB"
@ ["process","withName:convert_BAM2Pileup_SAMtools","memory","closure"]
- "retry_updater(1 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(1 GB, add, 5 GB, $task.attempt, memory)"
@ ["process","withName:create_IndelCandidate_SAMtools","memory","2"]
- "4 GB"
+ "6 GB"
@ ["process","withName:create_IndelCandidate_SAMtools","memory","3"]
- "7 GB"
+ "11 GB"
@ ["process","withName:create_IndelCandidate_SAMtools","memory","closure"]
- "retry_updater(1 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(1 GB, add, 5 GB, $task.attempt, memory)"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","2"]
- "16 GB"
+ "28 GB"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","closure"]
- "retry_updater(8 GB, exponential, 2, $task.attempt, memory)"
+ "retry_updater(8 GB, add, 20 GB, $task.attempt, memory)"
@ ["process","withName:run_sump_MuSE","memory","1"]
- "24 GB"
+ "16 GB"
@ ["process","withName:run_sump_MuSE","memory","closure"]
- "retry_updater(24 GB, add, 8 GB, $task.attempt, memory)"
+ "retry_updater(16 GB, add, 16 GB, $task.attempt, memory)"

test/configtest-F32.json

@ ["params","proc_resource_params","call_sSNV_Mutect2","memory"]
- "3 GB"
+ "2 GB"
@ ["params","proc_resource_params","call_sSNV_Mutect2","retry_strategy","memory","operand"]
- "3 GB"
+ "2 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","memory"]
- "16 GB"
+ "12 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","operand"]
- "2"
+ "20 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","strategy"]
- "exponential"
+ "add"
@ ["params","proc_resource_params","run_sump_MuSE","memory"]
- "48 GB"
+ "18 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","1"]
- "3 GB"
+ "2 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","2"]
- "6 GB"
+ "4 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","3"]
- "9 GB"
+ "6 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","closure"]
- "retry_updater(3 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(2 GB, add, 2 GB, $task.attempt, memory)"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","1"]
- "16 GB"
+ "12 GB"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","3"]
- "64 GB"
+ "52 GB"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","closure"]
- "retry_updater(16 GB, exponential, 2, $task.attempt, memory)"
+ "retry_updater(12 GB, add, 20 GB, $task.attempt, memory)"
@ ["process","withName:run_sump_MuSE","memory","1"]
- "48 GB"
+ "18 GB"
@ ["process","withName:run_sump_MuSE","memory","2"]
- "64 GB"
+ "34 GB"
@ ["process","withName:run_sump_MuSE","memory","3"]
- "64 GB"
+ "50 GB"
@ ["process","withName:run_sump_MuSE","memory","closure"]
- "retry_updater(48 GB, add, 16 GB, $task.attempt, memory)"
+ "retry_updater(18 GB, add, 16 GB, $task.attempt, memory)"

If the above changes are surprising, stop and determine what happened.

If the above changes are expected, there are two ways to fix this:

  1. Automatically: Post a comment starting with "/fix-tests" (without the quotes) and I will update the tests for you (you must review my work afterwards).
  2. Manually: Follow these steps on Confluence.
sorelfitzgibbon commented 6 months ago

/fix-tests

github-actions[bot] commented 6 months ago

Bleep bloop, I am a robot.

I have updated all of the failing tests for you with d63aa1b2e323de6c346cff1845d28949095c162e. You must review my work before merging this pull request!

github-actions[bot] commented 6 months ago

Bleep bloop, I am a robot.

Alas, some of the Nextflow configuration tests failed!

test/configtest-F16.json

@ ["params","proc_resource_params","call_sIndel_Manta","retry_strategy","memory","operand"]
- "3 GB"
+ "5 GB"
@ ["params","proc_resource_params","call_sSNV_Mutect2","memory"]
- "3 GB"
+ "2 GB"
@ ["params","proc_resource_params","convert_BAM2Pileup_SAMtools","retry_strategy","memory","operand"]
- "3 GB"
+ "5 GB"
@ ["params","proc_resource_params","create_IndelCandidate_SAMtools","retry_strategy","memory","operand"]
- "3 GB"
+ "5 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","operand"]
- "2"
+ "20 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","strategy"]
- "exponential"
+ "add"
@ ["params","proc_resource_params","run_sump_MuSE","memory"]
- "24 GB"
+ "16 GB"
@ ["params","proc_resource_params","run_sump_MuSE","retry_strategy","memory","operand"]
- "8 GB"
+ "16 GB"
@ ["process","withName:call_sIndel_Manta","memory","2"]
- "9 GB"
+ "11 GB"
@ ["process","withName:call_sIndel_Manta","memory","3"]
- "12 GB"
+ "16 GB"
@ ["process","withName:call_sIndel_Manta","memory","closure"]
- "retry_updater(6 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(6 GB, add, 5 GB, $task.attempt, memory)"
@ ["process","withName:call_sSNV_Mutect2","memory","1"]
- "3 GB"
+ "2 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","2"]
- "5 GB"
+ "4 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","3"]
- "7 GB"
+ "6 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","closure"]
- "retry_updater(3 GB, add, 2 GB, $task.attempt, memory)"
+ "retry_updater(2 GB, add, 2 GB, $task.attempt, memory)"
@ ["process","withName:convert_BAM2Pileup_SAMtools","memory","2"]
- "4 GB"
+ "6 GB"
@ ["process","withName:convert_BAM2Pileup_SAMtools","memory","3"]
- "7 GB"
+ "11 GB"
@ ["process","withName:convert_BAM2Pileup_SAMtools","memory","closure"]
- "retry_updater(1 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(1 GB, add, 5 GB, $task.attempt, memory)"
@ ["process","withName:create_IndelCandidate_SAMtools","memory","2"]
- "4 GB"
+ "6 GB"
@ ["process","withName:create_IndelCandidate_SAMtools","memory","3"]
- "7 GB"
+ "11 GB"
@ ["process","withName:create_IndelCandidate_SAMtools","memory","closure"]
- "retry_updater(1 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(1 GB, add, 5 GB, $task.attempt, memory)"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","2"]
- "16 GB"
+ "28 GB"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","closure"]
- "retry_updater(8 GB, exponential, 2, $task.attempt, memory)"
+ "retry_updater(8 GB, add, 20 GB, $task.attempt, memory)"
@ ["process","withName:run_sump_MuSE","memory","1"]
- "24 GB"
+ "16 GB"
@ ["process","withName:run_sump_MuSE","memory","closure"]
- "retry_updater(24 GB, add, 8 GB, $task.attempt, memory)"
+ "retry_updater(16 GB, add, 16 GB, $task.attempt, memory)"

test/configtest-F32.json

@ ["params","proc_resource_params","call_sSNV_Mutect2","memory"]
- "3 GB"
+ "2 GB"
@ ["params","proc_resource_params","call_sSNV_Mutect2","retry_strategy","memory","operand"]
- "3 GB"
+ "2 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","memory"]
- "16 GB"
+ "12 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","operand"]
- "2"
+ "20 GB"
@ ["params","proc_resource_params","run_LearnReadOrientationModel_GATK","retry_strategy","memory","strategy"]
- "exponential"
+ "add"
@ ["params","proc_resource_params","run_sump_MuSE","memory"]
- "48 GB"
+ "18 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","1"]
- "3 GB"
+ "2 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","2"]
- "6 GB"
+ "4 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","3"]
- "9 GB"
+ "6 GB"
@ ["process","withName:call_sSNV_Mutect2","memory","closure"]
- "retry_updater(3 GB, add, 3 GB, $task.attempt, memory)"
+ "retry_updater(2 GB, add, 2 GB, $task.attempt, memory)"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","1"]
- "16 GB"
+ "12 GB"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","3"]
- "64 GB"
+ "52 GB"
@ ["process","withName:run_LearnReadOrientationModel_GATK","memory","closure"]
- "retry_updater(16 GB, exponential, 2, $task.attempt, memory)"
+ "retry_updater(12 GB, add, 20 GB, $task.attempt, memory)"
@ ["process","withName:run_sump_MuSE","memory","1"]
- "48 GB"
+ "18 GB"
@ ["process","withName:run_sump_MuSE","memory","2"]
- "64 GB"
+ "34 GB"
@ ["process","withName:run_sump_MuSE","memory","3"]
- "64 GB"
+ "50 GB"
@ ["process","withName:run_sump_MuSE","memory","closure"]
- "retry_updater(48 GB, add, 16 GB, $task.attempt, memory)"
+ "retry_updater(18 GB, add, 16 GB, $task.attempt, memory)"

If the above changes are surprising, stop and determine what happened.

If the above changes are expected, there are two ways to fix this:

  1. Automatically: Post a comment starting with "/fix-tests" (without the quotes) and I will update the tests for you (you must review my work afterwards).
  2. Manually: Follow these steps on Confluence.
sorelfitzgibbon commented 6 months ago

/fix-tests

github-actions[bot] commented 6 months ago

Bleep bloop, I am a robot.

I have updated all of the failing tests for you with 6e2cbeb8554c65def2d319348acbc9fbef148811. You must review my work before merging this pull request!

sorelfitzgibbon commented 6 months ago

@yashpatel6 this is ready for review