The command bcl2fastq did not work after installing the bcl2fastq2 package.
This is the installation output:
(mancr) [tdeluca1@vacc-user1 20210630_181823]$ conda config --env --add channels conda-forge
(mancr) [tdeluca1@vacc-user1 20210630_181823]$ conda config --env --add channels bioconda
(mancr) [tdeluca1@vacc-user1 20210630_181823]$ conda install -c hcc bcl2fastq2
Collecting package metadata (current_repodata.json): done
Solving environment: done
## Package Plan ##
environment location: /users/t/d/tdeluca1/miniconda3/envs/mancr
added / updated specs:
- bcl2fastq2
The following packages will be downloaded:
package | build
---------------------------|-----------------
bcl2fastq2-2.20.0.422 | hac72e42_0 21.8 MB hcc
icu-58.2 | hf484d3e_1000 22.6 MB conda-forge
libboost-1.73.0 | h3ff78a5_11 13.9 MB
zstd-1.4.9 | ha95c52a_0 431 KB conda-forge
------------------------------------------------------------
Total: 58.7 MB
The following NEW packages will be INSTALLED:
bcl2fastq2 hcc/linux-64::bcl2fastq2-2.20.0.422-hac72e42_0
bzip2 conda-forge/linux-64::bzip2-1.0.8-h7f98852_4
icu conda-forge/linux-64::icu-58.2-hf484d3e_1000
libboost pkgs/main/linux-64::libboost-1.73.0-h3ff78a5_11
lz4-c conda-forge/linux-64::lz4-c-1.9.3-h9c3ff4c_0
python_abi conda-forge/linux-64::python_abi-3.9-2_cp39
zstd conda-forge/linux-64::zstd-1.4.9-ha95c52a_0
The following packages will be UPDATED:
ca-certificates pkgs/main::ca-certificates-2021.5.25-~ --> conda-forge::ca-certificates-2021.5.30-ha878542_0
The following packages will be SUPERSEDED by a higher-priority channel:
certifi pkgs/main::certifi-2021.5.30-py39h06a~ --> conda-forge::certifi-2021.5.30-py39hf3d152e_0
openssl pkgs/main::openssl-1.1.1k-h27cfd23_0 --> conda-forge::openssl-1.1.1k-h7f98852_0
Proceed ([y]/n)?
Downloading and Extracting Packages
icu-58.2 | 22.6 MB | ################################################################################################################################################################################################################################### | 100%
libboost-1.73.0 | 13.9 MB | ################################################################################################################################################################################################################################### | 100%
zstd-1.4.9 | 431 KB | ################################################################################################################################################################################################################################### | 100%
bcl2fastq2-2.20.0.42 | 21.8 MB | ################################################################################################################################################################################################################################### | 100%
Preparing transaction: done
Verifying transaction: done
Executing transaction: done
The bcl2fastq command fails when it does not find libboost version 1.67.0. The output of running bcl2fastq is:
(mancr) [tdeluca1@vacc-user1 20210630_181823]$ bcl2fastq --version
bcl2fastq: error while loading shared libraries: libboost_chrono.so.1.67.0: cannot open shared object file: No such file or directory
Installing libboost version 1.67.0 fixes the issue. Here is the output of installing 1.67.0 and running bcl2fastq:
(mancr) [tdeluca1@vacc-user1 20210630_181823]$ conda install libboost=1.67.0
Collecting package metadata (current_repodata.json): done
Solving environment: done
## Package Plan ##
environment location: /users/t/d/tdeluca1/miniconda3/envs/mancr
added / updated specs:
- libboost=1.67.0
The following packages will be downloaded:
package | build
---------------------------|-----------------
libboost-1.67.0 | h46d08c1_4 13.0 MB
------------------------------------------------------------
Total: 13.0 MB
The following packages will be DOWNGRADED:
libboost 1.73.0-h3ff78a5_11 --> 1.67.0-h46d08c1_4
Proceed ([y]/n)?
Downloading and Extracting Packages
libboost-1.67.0 | 13.0 MB | ################################################################################################################################################################################################################################### | 100%
Preparing transaction: done
Verifying transaction: done
Executing transaction: done
(mancr) [tdeluca1@vacc-user1 20210630_181823]$ bcl2fastq --version
BCL to FASTQ file converter
bcl2fastq v2.20.0.422
Copyright (c) 2007-2017 Illumina, Inc.
The command
bcl2fastq
did not work after installing the bcl2fastq2 package.This is the installation output:
The bcl2fastq command fails when it does not find libboost version 1.67.0. The output of running bcl2fastq is:
Installing libboost version 1.67.0 fixes the issue. Here is the output of installing 1.67.0 and running bcl2fastq: