Is it possible to handle long-read RNA-seq data with VAST-TOOL?

vastgroup / vast-tools

A toolset for profiling alternative splicing events in RNA-Seq data.

MIT License

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Is it possible to handle long-read RNA-seq data with VAST-TOOL? #118

Closed happypiggyzjx closed 3 months ago

happypiggyzjx commented 7 months ago

Dear VAST-TOOL Software Development Team，

Greetings! Thank you very much for developing such a great software, and now I have a small question for you: is it possible to handle long-read RNA-seq data with this software? If so, is there anything I should be aware of? Thank you again for taking time out of your busy schedule to read this email, your professional reply really means a lot to me.

Sincerely， Happy Piggy

mirimia commented 3 months ago

Hello,

It is technically possible if provided in FQ format, but not recommended.

If you want a similar quantification, exon-based, I recommend using insplico.

Cheers Manu