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vdemichev / DiaNN

DIA-NN - a universal automated software suite for DIA proteomics data analysis.
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67 precursors, 0 proteins identified #873

Open Sarahlal99 opened 7 months ago

Sarahlal99 commented 7 months ago

Dear Vadim I performed a DiaNN analysis on my MDA-MB-231 vesicular protein samples. However, at the end of the identification process, my protein group file was empty, even though I observed that it identified precursors that did not correspond to proteins. I'm really not sure what the issue is, so if you have any suggestions please, they would be welcome. Attached is the report.

Thank you for your response, and I wish you a good day.

Kind regards

Sarah report.log.txt

vdemichev commented 6 months ago

Hi Sarah,

Simply no proteins passing the 1% FDR filter. First thing to try is (i) reduce the search space as much as possible, i.e. no variable modifications, etc (ii) use a public spectral library if available

Best, Vadim