Dear author,
For some genes, when I first run the hyphy relax command:
/data/01/p1/user157/software/hyphy/bin/hyphy relax --alignment /data/01/p1/user157/Bathyergidae-sociaty/2024812.newcactus/03.proteincoding-selected-analysis/01.getgene/07.deletestopcodon/evm.model.ptg000026l.297.fa --tree /data/01/p1/user157/Bathyergidae-sociaty/2024812.newcactus/03.proteincoding-selected-analysis/01.getgene/13.hyphy/02.relax/00.mark.tree/evm.model.ptg000026l.297.fa.treefile.mark --test FG1 --reference FG2 --output /data/01/p1/user157/Bathyergidae-sociaty/2024812.newcactus/03.proteincoding-selected-analysis/01.getgene/13.hyphy/02.relax/01.socialtest.relax.out/evm.model.ptg000026l.297.json > /data/01/p1/user157/Bathyergidae-sociaty/2024812.newcactus/03.proteincoding-selected-analysis/01.getgene/13.hyphy/02.relax/01.socialtest.relax.out/evm.model.ptg000026l.297.log 2>&1
The error occurs in the log file:
Error:
Internal error
Constrained optimization failed, since a starting point within the domain specified for the variables couldn't be found.
Set it by hand, or check your constraints for compatibility.
Failed constraint: relax.ge.omega2:=1/0.3652980831447958 must be in [0,10000]. Current value = 1.
Then I rerun the the same command, the error disappears and the dN/dS distribution for models is shown in the log file:
### Fitting the alternative model to test K != 1
* Log(L) = -941.31, AIC-c = 1969.90 (42 estimated parameters)
* Relaxation/intensification parameter (K) = 0.01
* The following rate distribution was inferred for **test** branches
| Selection mode | dN/dS |Proportion, %| Notes |
|-----------------------------------|---------------|-------------|-----------------------------------|
| Negative selection | 0.976 | 39.361 | |
| Negative selection | 0.976 | 37.942 | Collapsed rate class |
| Neutral evolution | 1.000 | 22.697 | Collapsed rate class |
* The following rate distribution was inferred for **reference** branches
| Selection mode | dN/dS |Proportion, %| Notes |
|-----------------------------------|---------------|-------------|-----------------------------------|
| Negative selection | 0.045 | 39.361 | |
| Negative selection | 0.088 | 37.942 | |
| Neutral evolution | 1.000 | 22.697 | |
The difference for two runs is obvious and which result could I trust?
The input files are attached.
Could you give me any suggestions?
Looking forward with your reply.
Dear author, For some genes, when I first run the hyphy relax command:
/data/01/p1/user157/software/hyphy/bin/hyphy relax --alignment /data/01/p1/user157/Bathyergidae-sociaty/2024812.newcactus/03.proteincoding-selected-analysis/01.getgene/07.deletestopcodon/evm.model.ptg000026l.297.fa --tree /data/01/p1/user157/Bathyergidae-sociaty/2024812.newcactus/03.proteincoding-selected-analysis/01.getgene/13.hyphy/02.relax/00.mark.tree/evm.model.ptg000026l.297.fa.treefile.mark --test FG1 --reference FG2 --output /data/01/p1/user157/Bathyergidae-sociaty/2024812.newcactus/03.proteincoding-selected-analysis/01.getgene/13.hyphy/02.relax/01.socialtest.relax.out/evm.model.ptg000026l.297.json > /data/01/p1/user157/Bathyergidae-sociaty/2024812.newcactus/03.proteincoding-selected-analysis/01.getgene/13.hyphy/02.relax/01.socialtest.relax.out/evm.model.ptg000026l.297.log 2>&1
The error occurs in the log file:
Then I rerun the the same command, the error disappears and the dN/dS distribution for models is shown in the log file:
The difference for two runs is obvious and which result could I trust?
The input files are attached.
Could you give me any suggestions? Looking forward with your reply.
Best wishes, Na Wan evm.model.ptg000026l.297.json evm.model.ptg000026l.297.log evm.model.ptg000026l.297.fa.treefile.mark.txt evm.model.ptg000026l.297.fa.txt