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weissmanlab / magic

Minimal Assumption Genomic Inference of Coalescence
MIT License
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Minimum sequencing depth when using single individual to infer demographic. #7

Open xiekunwhy opened 3 years ago

xiekunwhy commented 3 years ago

Hi,

If there is only one individual, how depth I need to sequence (whole genome sequencing) if I want to get reliability results using magic, 5X, 10X, 20X or 30X ?

Best, Kun

weissman commented 3 years ago

Probably 30X. You want at least 80-90% of the sites to have high-quality calls, and you don't want there to be big biases in what gets called.

On Fri, Mar 5, 2021 at 1:39 AM xiekunwhy notifications@github.com wrote:

Hi,

If there is only one individual, how depth I need to sequence (whole genome sequencing) if I want to get reliability results using magic, 5X, 10X, 20X or 30X ?

Best, Kun

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xiekunwhy commented 3 years ago

I see, thank you for your answers.