Data and code related to the paper:cycling cancer persister cells arise from lineages with distinct programs (https://www.nature.com/articles/s41586-021-03796-6) For any addiotnal code requests or questions please email me (yoren@broadinstitute.org).
This project contains two directories Code and Data.
The Code directory contains:
1)R script 10X_WTA_dialOut.md- this script creates a cell-barcode:lineage-barcode directory based on a dialout-PCR of watermelon mammalian lines
2)R script plasmid_pool_barcode_dialOut- this script is used to assess complexity of a watermelon plasmid pool. It is typically used on a dialout-PCR of a bacterial maxi prep
The Data directory contains:
1)V3 Seurat R objects pc9_time_course.rds- transcriptome of PC9-watermelon cell lines across osimertinib treatment. Lineage barcodes are marked in the metadata file associated with the Seurat objects (dim: 22166 56419).
2)V3 Seurat R objects pooled_persisters.rds-transcriptome of multiple watermelon cell lines at day 0 and 10 of drug treatment. Cell line identity is marked in the metadata file associated with the Seurat object (dim: 23168 50735).
Both objects can be loaded using the readRDS R function if the Seurat library is loaded