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zitmen / thunderstorm

ThunderSTORM: a comprehensive ImageJ plugin for SMLM data analysis and super-resolution imaging
http://zitmen.github.io/thunderstorm/
GNU General Public License v3.0
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Base level offset #71

Open davepypy opened 3 years ago

davepypy commented 3 years ago

Hi,

we're using a Hamamatsu ORCA Flash 4.0 V2 sCMOS camera for which the stated e-/ADU conversion factor is 0.46 e-/count and "dark offset" is 100 counts:

Screenshot

However, if these values are entered into the camera parameter settings, ThunderSTORM complains that the base level offset is higher than the signal:

Screenshot

What might be the issue here? Is it correct that the "dark offset" has to be given in ADU counts and not electrons?

Thanks and best regards, Dave

kclwcic commented 11 months ago

Wondering if there was a resolution to this - Dave, did you find the answer?

cleterrier commented 11 months ago

Dark offset has to be entered in ADU i.e. 100

Christophe

Le mer. 2 août 2023, 10:24, Wohl Cellular Imaging Centre < @.***> a écrit :

Wondering if there was a resolution to this - Dave, did you find the answer?

— Reply to this email directly, view it on GitHub https://github.com/zitmen/thunderstorm/issues/71#issuecomment-1661734993, or unsubscribe https://github.com/notifications/unsubscribe-auth/AAIDLC2MBJW25U5PZUDANSTXTIE6NANCNFSM4SIW5IQA . You are receiving this because you are subscribed to this thread.Message ID: @.***>