Hi all,
I downloaded the original bam file from SRA database and ran bamtofastq with "--traceback" twice, each time getting this error:
bamtofastqerror: Not a valid read pair: false, false
Please contact patrick@10xgenomics.com for assistance. Please re-run with --traceback and include stack trace with an error report
see below for more details:
==========================
Not a valid read pair: false, false
0: failure::backtrace::internal::InternalBacktrace::new
at execroot/home/registry/src/github.com-1ecc6299db9ec823/failure-0.1.8/execroot/home/registry/src/github.com-1ecc6299db9ec823/failure-0.1.8/src/backtrace/internal.rs:46
failure::backtrace::Backtrace::new
at execroot/home/registry/src/github.com-1ecc6299db9ec823/failure-0.1.8/execroot/home/registry/src/github.com-1ecc6299db9ec823/failure-0.1.8/src/backtrace/mod.rs:121
1: <failure::error::error_impl::ErrorImpl as core::convert::From<F>>::from
at execroot/exec/external/bamtofastq/execroot/home/registry/src/github.com-1ecc6299db9ec823/failure-0.1.8/src/error/error_impl.rs:19
<failure::error::Error as core::convert::From<F>>::from
at execroot/exec/external/bamtofastq/execroot/home/registry/src/github.com-1ecc6299db9ec823/failure-0.1.8/src/error/mod.rs:36
failure::error_message::err_msg
at execroot/exec/external/bamtofastq/execroot/home/registry/src/github.com-1ecc6299db9ec823/failure-0.1.8/src/error_message.rs:12
bamtofastq::FormatBamRecords::bam_rec_to_ser
at execroot/exec/external/bamtofastq/src/main.rs:494
2: bamtofastq::proc_double_ended
at execroot/exec/external/bamtofastq/src/main.rs:1185
bamtofastq::inner
at execroot/exec/external/bamtofastq/src/main.rs:1110
3: bamtofastq::go
at execroot/exec/external/bamtofastq/src/main.rs:1020
bamtofastq::main
at execroot/exec/external/bamtofastq/src/main.rs:977
4: core::ops::function::FnOnce::call_once
at /mnt/build/toolchains/tools/rust/1.53.0/lib/rustlib/src/rust/library/core/src/ops/function.rs:227
std::sys_common::backtrace::__rust_begin_short_backtrace
at /mnt/build/toolchains/tools/rust/1.53.0/lib/rustlib/src/rust/library/std/src/sys_common/backtrace.rs:125
5: main
6: __libc_start_main
7: <unknown>
Hi all, I downloaded the original bam file from SRA database and ran bamtofastq with "--traceback" twice, each time getting this error:
What should I do next ?