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10XGenomics / bamtofastq

Convert 10x BAM files to the original FASTQs compatible with 10x pipelines
MIT License
59 stars 6 forks source link

Make the tool work with "cellranger vdj" bam outputs #108

Open sralchemab opened 1 year ago

sralchemab commented 1 year ago

It would be great if the tool could produce a FastQ file from the VDJ pipeline BAM files (e.g. all_contig.bam), including the usage of the --bx-list option as well.

Thanks in advance.

apredeus commented 5 months ago

I agree, it would be great to have a tool like that. Often users submit BAM files to GEO/SRA, and their decoding is, to put it mildly, often inconsistent. E.g. in this project, there are 14 10x VDJ experiments, 12 of which were decoded as paired-end reads, and 2 as single-end read for some reason.

Are there any tools that decode VDJ BAMs to paired-end reads correctly? Also, why is there a cap on how many reads per barcode are outputted to the all_contig.bam file? It's so great that a regular 10x BAM file contains all of the reads, makes uploading a BAM or original fastq files truly interchangeable. However it causes lots of confusion, because most of the raw read upload systems allow you to upload a BAM instead of fastq files.