10XGenomics / cellranger

10x Genomics Single Cell Analysis
https://www.10xgenomics.com/support/software/cell-ranger
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Cellranger count: Found two feature definitions with the same ID #130

Closed capsellabursa closed 2 years ago

capsellabursa commented 3 years ago

Almost the same issue as #39 in cellranger-6.0.1 The issue occurs after successful work mkref during count, the GTF is custom, but based on an example from the best practices. The features gene don't have transcript_id

Here is the error:

Found two feature definitions with the same ID: 
(ID: b'rna-NC_020754.1:1..67'; name: rna-NC_020754.1:1..67; type: Gene Expression) and (ID: b'rna-NC_020754.1:1..67'; name: 
rna-NC_020754.1:1..67; type: Gene Expression). 
All feature IDs must be distinct.

And part of GTF:

NC_020754.1 StringTie_RefSeq    exon    1   67  .   +   .   gene_id "rna-NC_020754.1:1..67"; transcript_id "rna-NC_020754.1:1..67";
NC_020754.1 StringTie   gene    1   67  .   +   .   gene_id "rna-NC_020754.1:1..67";
NC_020754.1 StringTie   gene    67  1029    .   +   .   gene_id "s-rRNA"; Note "12SribosomalRNA"; gbkey "rRNA"; product "s-rRNA";
NC_020754.1 StringTie_RefSeq    exon    67  1029    .   +   .   gene_id "s-rRNA"; transcript_id "s-rRNA"; Note "12SribosomalRNA"; gbkey "rRNA"; product "s-rRNA";

How to fix this problem?

capsellabursa commented 3 years ago

@pryvkin10x

pryvkin10x commented 3 years ago

Hello,

Thanks for reaching out. In order to get a timely response to your issue, please reach out to support@10xgenomics.com and they'll help you out.

trum994 commented 2 years ago

Hello - is there a solution to this you can share ?