10XGenomics / cellranger

10x Genomics Single Cell Analysis
https://www.10xgenomics.com/support/software/cell-ranger
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The number of read1 and read2 is inconsistent #160

Closed yingHH closed 8 months ago

yingHH commented 2 years ago

Hi, I got ARC (snRNA + snATAC) data, but got an error when running cellranger-arc count:

[runtime] (failed)          ID.4567.SC_ATAC_GEX_COUNTER_CS.SC_ATAC_GEX_COUNTER._ATAC_MATRIX_COMPUTER.MAKE_ATAC_SHARDS

[error] Pipestance failed. Error log at:
4567/SC_ATAC_GEX_COUNTER_CS/SC_ATAC_GEX_COUNTER/_ATAC_MATRIX_COMPUTER/MAKE_ATAC_SHARDS/fork0/chnk3-ud2dd5584fc/_errors

Log message:
Input FASTQ file ended prematurely: file: "4577-1-atac-4_S4_L003_R3_001.fastq.gz", line: 154827

I checked the corresponding FASTQ files and found that the number of read1 (R1) and read2 (R3) were inconsistent. I would like to know if the number of read1 and read2 must be the same so that I can determine if the data I get is qualified.

Many thanks!