Closed yingHH closed 8 months ago
Hi, I got ARC (snRNA + snATAC) data, but got an error when running cellranger-arc count:
ARC (snRNA + snATAC)
cellranger-arc count
[runtime] (failed) ID.4567.SC_ATAC_GEX_COUNTER_CS.SC_ATAC_GEX_COUNTER._ATAC_MATRIX_COMPUTER.MAKE_ATAC_SHARDS [error] Pipestance failed. Error log at: 4567/SC_ATAC_GEX_COUNTER_CS/SC_ATAC_GEX_COUNTER/_ATAC_MATRIX_COMPUTER/MAKE_ATAC_SHARDS/fork0/chnk3-ud2dd5584fc/_errors Log message: Input FASTQ file ended prematurely: file: "4577-1-atac-4_S4_L003_R3_001.fastq.gz", line: 154827
I checked the corresponding FASTQ files and found that the number of read1 (R1) and read2 (R3) were inconsistent. I would like to know if the number of read1 and read2 must be the same so that I can determine if the data I get is qualified.
FASTQ
read1 (R1)
read2 (R3)
read1
read2
Many thanks!
Hi, I got
ARC (snRNA + snATAC)
data, but got an error when runningcellranger-arc count
:I checked the corresponding
FASTQ
files and found that the number ofread1 (R1)
andread2 (R3)
were inconsistent. I would like to know if the number ofread1
andread2
must be the same so that I can determine if the data I get is qualified.Many thanks!