Unable to auto-detect chemistry.

[fjptwenger]

2022-06-02 15:11:02 [runtime] (chunks_complete) ID.S7D.SC_RNA_COUNTER_CS.SC_RNA_COUNTER.SC_RNA_ANALYZER.CHOOSE_DIMENSION_REDUCTION
2022-06-02 15:11:06 [runtime] (progress)        ID.S7D.SC_RNA_COUNTER_CS.SC_RNA_COUNTER.CHEMISTRY_DETECTOR.DETECT_CHEMISTRY.fork0.chnk0: Building transcriptome...
2022-06-02 15:12:09 [runtime] (progress)        ID.S7D.SC_RNA_COUNTER_CS.SC_RNA_COUNTER.CHEMISTRY_DETECTOR.DETECT_CHEMISTRY.fork0.chnk0: Building kmer index...
2022-06-02 15:13:04 [runtime] (progress)        ID.S7D.SC_RNA_COUNTER_CS.SC_RNA_COUNTER.CHEMISTRY_DETECTOR.DETECT_CHEMISTRY.fork0.chnk0: Mapping reads...
2022-06-02 15:13:18 [runtime] (failed)          ID.S7D.SC_RNA_COUNTER_CS.SC_RNA_COUNTER.CHEMISTRY_DETECTOR.DETECT_CHEMISTRY

[error] Unable to auto-detect chemistry. Sample def 0/TTS7D: Fraction of barcodes on whitelist was at best 0.23%, while we expected at least 10.00% for one of the chemistries.

read1.gz

@A01045:372:HNTLCDSXY:1:1101:20654:1063 1:N:0:ATTACTCG+NGCTCTGA
ANCCGCCTCACTGGCCTGCGAGACCTTAGAGGTAGCGGTGACAAGCCTGGTTCTGCAAGTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
F#FFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,:FFFFFFFFFFF:,F,:FFFFFFF::FF:,FFFFFFF:,FFFF:FF:FFF:FF:FFFFFFFFFF,F:F:::FF:F:FFFFF:F,FF,,F,

read2.gz

@A01045:372:HNTLCDSXY:1:1101:20654:1063 2:N:0:ATTACTCG+NGCTCTGA
NAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
#FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFF:F,:FFFFFFF:FFFFFFFFFFFFFFF:FF:FFFFFFF:FFFFFF::FFFFFFFFFFFFFFF:FFFFF:::F:F:FFF:FFFF,FFFFFFF,FFF::FFFF:FF:FFFF:FF,:F:

[zhangls9791]

Hi, did you solve it later?

[evolvedmicrobe]

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