Closed tangybat closed 1 year ago
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I am trying to run velocyto to generate loom files needed for scvelo analysis. It appears that I lack the "possorted_genome_bam.bam" file needed for velocyto that is supposed to be located in the cellranger output folder. Here is my code: (I was running it in a cluster)
My code generated this error:
2023-04-22 11:18:39,382 - DEBUG - Using logic: Default 2023-04-22 11:18:39,402 - INFO - Read 2987 cell barcodes from /cellranger_output/NOThighp_10/outs/filtered_feature_bc_matrix/barcodes.tsv.gz 2023-04-22 11:18:39,402 - DEBUG - Example of barcode: AAACATGCAACTAACT and cell_id: NOThighp_10:AAACATGCAACTAACT-1 2023-04-22 11:18:39,414 - DEBUG - Peeking into /cellranger_output/NOThighp_10/outs/possorted_genome_bam.bam [E::hts_open_format] Failed to open file "/cellranger_output/NOThighp_10/outs/possorted_genome_bam.bam" : No such file or directory Traceback (most recent call last):
The cellranger output folder has an outs subfolder that contains "atac_possorted_bam.bam" and "gex_possorted_bam.bam" files but no possorted_genome_bam.bam file. Am I missing this file in my folder?