Dehash fastq files

[royfrancis]

I have fastq files from a hashed 10X VDJ library that looks like this:

PBMC_1_3_1_TCR_S5_L001_I1_001.fastq.gz
PBMC_1_3_1_TCR_S5_L001_R2_001.fastq.gz
PBMC_1_3_1_TCR_S5_L002_R1_001.fastq.gz
PBMC_1_3_1_TCR_S5_L001_I2_001.fastq.gz
PBMC_1_3_1_TCR_S5_L002_I1_001.fastq.gz
PBMC_1_3_1_TCR_S5_L002_R2_001.fastq.gz
PBMC_1_3_1_TCR_S5_L001_R1_001.fastq.gz
PBMC_1_3_1_TCR_S5_L002_I2_001.fastq.gz

This is one library that has 3 samples hashed and pooled together. Is it possible to dehash these fastq files into three separate fastq files by sample? I am looking for dehashing at fastq level and not at count level.

[evolvedmicrobe]

This is our current recommended workflow for this.