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10XGenomics / cellranger

10x Genomics Single Cell Analysis
https://www.10xgenomics.com/support/software/cell-ranger
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Duplicate FASTQs found when use cellranger count for Feature Barcode Analysis #225

Closed mengchengyao closed 8 months ago

mengchengyao commented 10 months ago

Hi, thank you for great tool for single cell Feature Barcode Analysis,. Now, I download fastq files from ncbi with sra formats, which combined gene expression fastq file, vdj fastq file and antigen Capture fastq file for only one fastq file , and it difficult to split the fastq file, so I analysised single cell gene expression and feature barcode with this fastq file by cellranger count or multi, hower cellranger could not work with error of 'Duplicate FASTQs found between Sample'.

Could you help me pleases? How can I sovle this problem?

Bests

evolvedmicrobe commented 10 months ago

Please contact support@10xgenomics.com - you likely will need to split up your FASTQ files into separate ones for each library.

mengchengyao commented 10 months ago

ok thanks