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10XGenomics / cellranger

10x Genomics Single Cell Analysis
https://www.10xgenomics.com/support/software/cell-ranger
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Anyone used for unmodified HTO primers vs. modified HTO primers? #69

Closed Jimmyyun closed 4 years ago

Jimmyyun commented 4 years ago

Hi I was following the old cell hashing protocol, which was without phosphorothioate modification in HTO cDNA PCR additive primer, SI-PCR primer, and TruSeq D701_s primer. However, it seems now all three primers use the phosphorothioate modification. I understand the purpose of using that modification that is for inhibiting nuclease degradation. Has anyone seen the difference in the Cell Hashing case? Does it make a difference enough to get much better quality data? Best

evolvedmicrobe commented 4 years ago

Hi @Jimmyyun,

10X has announced at AGBT we'll release a cell multiplexing protocol, but at present we do not have a supported solution. I'd encourage you to reach out to the relevant method developers for more help with this issue.

Warm wishes, N