[error] FASTQ files differ in number. Exiting pipeline.

[yejg2017]

When I run cellranger count with fastsqs: DME-6_S1_L001_I1_001.fastq.gz DME-6_S1_L001_R1_001.fastq.gz DME-6_S1_L001_R2_001.fastq.gz DME-6_S2_L001_R1_001.fastq.gz DME-6_S2_L001_R2_001.fastq.gz DME-6_S3_L001_I1_001.fastq.gz DME-6_S3_L001_R1_001.fastq.gz DME-6_S3_L001_R2_001.fastq.gz DME-6_S4_L001_R1_001.fastq.gz DME-6_S4_L001_R2_001.fastq.gz And I got this error,but the tutorial tell me that the I1 index file is optional,I do not how to fixed this issue,Counld you help me?Thanks

[evolvedmicrobe]

The fastq reqd file types need to be consistent, so either all files must have an I1 or none of them should. Feel free to reach out to support@10xgenomics.com for more information.

[yejg2017]

Thanks!And I want to ask for help for an another problem.When I run cellranger-atac count with fastqs: CAT-08_S1_L001_I1_001.fastq.gz CAT-08_S1_L001_R1_001.fastq.gz CAT-08_S1_L001_R2_001.fastq.gz CAT-08_S1_L001_R3_001.fastq.gz CAT-08_S2_L001_I1_001.fastq.gz CAT-08_S2_L001_R1_001.fastq.gz CAT-08_S2_L001_R2_001.fastq.gz CAT-08_S2_L001_R3_001.fastq.gz CAT-08_S3_L001_I1_001.fastq.gz CAT-08_S3_L001_R1_001.fastq.gz CAT-08_S3_L001_R2_001.fastq.gz CAT-08_S3_L001_R3_001.fastq.gz CAT-08_S4_L001_I1_001.fastq.gz CAT-08_S4_L001_R1_001.fastq.gz CAT-08_S4_L001_R2_001.fastq.gz CAT-08_S4_L001_R3_001.fastq.gz

The log file told me the error: [error] Input FASTQ file is not gzipped but filename has CAT-08_S1_L001_R2_001.fastq.gz suffix: .gz