DaPars_Extract_Anno.py - coordinates in output BED file (and 'Loci' column) are shifted 1 nucleotide from source transcript

3UTR / DaPars2

Dynamics analysis of Alternative PolyAdenylation from RNA-seq

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DaPars_Extract_Anno.py - coordinates in output BED file (and 'Loci' column) are shifted 1 nucleotide from source transcript #8

Closed SamBryce-Smith closed 2 years ago

SamBryce-Smith commented 2 years ago

This is a duplicate of ZhengXia/dapars#16 in the DaPars repo, as DaPars_Extract_Anno.py is identical between the repos.

TL;DR:

+ strand: DaPars BED/'Loci' 3'UTR start is 1 nt downstream of the actual annotated start - strand: Dapars BED/'Loci' 3'end of the 3'UTR is 1 nt upstream of the actual annotated end

3UTR commented 2 years ago

We have fixed this issue and thus close it.