3dem / relion

Image-processing software for cryo-electron microscopy
https://relion.readthedocs.io/en/latest/
GNU General Public License v2.0
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error in extraction after manual picking after autopicking #1126

Open Gia1975 opened 3 months ago

Gia1975 commented 3 months ago

Dear All,

After autopicking, I wanted to manually curate the particles (ManualPick job) but when I extract the saved particles, I get this error:

Do you know where is coming from ?

I am running Relion4 on Ubuntu 18.

With many thanks,

GIA

WARNING: ManualPick/job072/micrographs_selected.star seems to be from a previous version of Relion. Attempting conversion... You should make sure metadata in the optics group table after conversion is correct. in: /data/software/relion4/relion/src/jaz/single_particle/io/star_converter.cpp, line 14 ERROR: StarConverter::convert_3p0_particlesTo_3p1: Star file is already at version 3.0001 === Backtrace === /data/software/relion4/relion/build/bin/relion_preprocess(_ZN11RelionErrorC1ERKNSt7cxx1112basic_stringIcSt11char_traitsIcESaIcEEES7_l+0x77) [0x5626fe4b3067] /data/software/relion4/relion/build/bin/relion_preprocess(_ZN13StarConverter27convert_3p0_particlesTo_3p1ERK13MetaDataTableRS0_S3_NSt7cxx1112basic_stringIcSt11char_traitsIcESaIcEEEb+0x302) [0x5626fe517652] /data/software/relion4/relion/build/bin/relion_preprocess(_ZN16ObservationModel10loadSafelyENSt7cxx1112basic_stringIcSt11char_traitsIcESaIcEEERS_R13MetaDataTableS5_ib+0x3a7) [0x5626fe4fd437] /data/software/relion4/relion/build/bin/relion_preprocess(_ZN13Preprocessing10initialiseEv+0x16d) [0x5626fe4dcf0d] /data/software/relion4/relion/build/bin/relion_preprocess(main+0x49) [0x5626fe4a99b9] /lib/x86_64-linux-gnu/libc.so.6(libc_start_main+0xe7) [0x7f1efc668c87] /data/software/relion4/relion/build/bin/relion_preprocess(_start+0x2a) [0x5626fe4aa2ca]

ERROR: StarConverter::convert_3p0_particlesTo_3p1: Star file is already at version 3.0001This is a template for reporting bugs. Please fill in as much information as you can.

Gia1975 commented 3 months ago

Actually,

I get the same identical error when training Topaz with particles (along with their corresponding micrographs) that have been curated using the manualpicker... So there must be something with the manual picker that screw up .star files...