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3dem / relion

Image-processing software for cryo-electron microscopy
https://relion.readthedocs.io/en/latest/
GNU General Public License v2.0
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Take anisotropic mag info to motioncorr2 ? #549

Closed FFTMing closed 4 years ago

FFTMing commented 4 years ago

The higher order anisotropic mag and aberration correction are amazing!

As the anisotropic mag is pretty consistent under similar data collection conditions, I'm wondering if we could take the info from RELION 3.1 to UCSF MotionCorr2. Where can I find the angle def etc? Does it use the same angle etc convention as it's in Niko's mag_distortion_estimate? MotionCorr2 follows mag_distortion_estimate conventions.

Thanks,

biochem-fan commented 4 years ago

No. RELION's preferred way is to apply correction during reconstruction (Refine3D), not during initial motion correction. This way, you can reduce the number of interpolations. If you apply -Mag flag in UCSF MotionCor2, you cannot run Polishing.

The information is in rlnMagMatrix_00 to rlnMagMatrix_11, which corresponds to the Matrix M in our aberration preprint. It is more general than mag_distortion_estimate because it can represent a skew component.