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3dem / relion

Image-processing software for cryo-electron microscopy
https://relion.readthedocs.io/en/latest/
GNU General Public License v2.0
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Issues to get a proper Autopick-2D classification #770

Closed Ulvetanna3 closed 3 years ago

Ulvetanna3 commented 3 years ago

Hi,

I'm trying to Autopick (Laplacian) a set of elongated particles that are around 150A long. I manage to get a nice 2D classes in Cryosparc but, since I have preferential orientation, people recommend me to use Relion to redo my dataset analysis. But I'm really stuck, I have no experience with Relion and I don't manage to get any good autopicking that lead me to a 2D classification (even a preliminar one to re do the picking after), I just get black images in the 2D classification, even when I check the autopick and it doesn't look that bad.

Honestly idk what to put here about the parameters that I'm using because I tried a bunch but blindly (since I have no idea neither experience).

I was hoping someone could give me some suggestions that help me to overcome that point because I'm desperated.

Thanks!

biochem-fan commented 3 years ago

Please post data processing questions to CCPEM, not here.