Closed Leotrosa closed 9 months ago
-Doing a new initial model (this works ok) and then using this model for a refinement (gives same error)
Are you sure your mask and the new initial model are consistently aligned?
With the refinement comming from the initial model I did not even use a mask, and it crashed anyway with the refinement after the 3D classification, I double checked before launching and they are well aligned, yes
What happens if you make a reference by relion_reconstruct
on the subtracted particles?
Hi just tried doing a relion_reconstruct on the subsract particles (which works ok) but the refine crashes with the same error
However, checking the relion_reconstruct result, it resulted in a sphere full of noise and no density inside. Does that mean the problem is potentially back in the subtraction job?
You have to low pass filter the reconstructed map (or reconstruct half sets separately and run postprocess) before visually inspecting it.
Thanks, the reconstruct map looks ok after lowpass filtering
Hi all, In Relion 4, I made a mask on the region of interest of my map and performed a subtract job, centering images on mask after subtraction. Then I try to run a refinement using the subracted particles, but the same error appears:
ERROR: No orientation was found as better than any other.
A particle image was compared to the reference and resulted in all-zero weights (for all orientations). This should not happen, unless your data has very special characteristics. This has historically happened for some lower-precision calculations, but multiple fallbacks have since been implemented. Please report this error to the relion developers at github.com/3dem/relion/issues
I tried different strategies: -Using the original map as a reference with the reference mask -Doing a new initial model (this works ok) and then using this model for a refinement (gives same error) -Doing a 3D classification to pre-align particles (this works fne) before doing a refinement (gives same error)
Environment:
Dataset:
Job options:
note.txt
in the job directory):=================