The following items are missing and I think they would be useful to have:
[x] The description present in lines 9-10 from the google-sheet
-- TO-DO
[x] The description present in lines 34-35 from the google-sheet
-- TO-DO
The following items are missing and might need to be removed from google sheet:
[x] The note present in line 44 in the google-sheet
[x] Please modify this table s follows (based on discussion between Caterina, Andrea and Alistair on 2/14/2022):
Summary
This table is optional and is designed to be used in the case of multiplexed FISH experiments (i.e., MERFISH) in which the final localization of a bright DNA or RNA Spot of interest results from the combination of multiple individual localization events (e.g., by combining particles detected and localized in separate images). In such a case the final Spot localization data is recorded in the core DNA Spot/Trace Data table while the underlying primary localization data can be recorded by using this table as shown for example RNA Spots in the example below.
This table is indexed by Loc_ID and it has a mandatory Spot_ID column that is used to link individual localization events to the resulting Spot.
Example 1: DNA spots detected with multiplexed barcodes
Loc_ID Spot_ID x y z hyb fluor brightness fit_quality
1 1 2342 2354 545 2 cy3 1003 .83
2 1 2342 2354 545 2 cy5 2000 .93
3 1 2342 2354 545 3 cy5 1233 .85
4 2 3345 5432 654 3 cy3 2324 .95
5 2 3345 5432 654 3 cy5 2324 .95
6 NA 4345 432 100 4 cy3 2324 .95
7 2 3345 5432 654 4 cy3 2324 .9
The following items are missing and I think they would be useful to have:
The following items are missing and might need to be removed from google sheet:
[x] The note present in line 44 in the google-sheet
[x] Please modify this table s follows (based on discussion between Caterina, Andrea and Alistair on 2/14/2022): Summary This table is optional and is designed to be used in the case of multiplexed FISH experiments (i.e., MERFISH) in which the final localization of a bright DNA or RNA Spot of interest results from the combination of multiple individual localization events (e.g., by combining particles detected and localized in separate images). In such a case the final Spot localization data is recorded in the core DNA Spot/Trace Data table while the underlying primary localization data can be recorded by using this table as shown for example RNA Spots in the example below. This table is indexed by Loc_ID and it has a mandatory Spot_ID column that is used to link individual localization events to the resulting Spot. Example 1: DNA spots detected with multiplexed barcodes Loc_ID Spot_ID x y z hyb fluor brightness fit_quality 1 1 2342 2354 545 2 cy3 1003 .83 2 1 2342 2354 545 2 cy5 2000 .93 3 1 2342 2354 545 3 cy5 1233 .85 4 2 3345 5432 654 3 cy3 2324 .95 5 2 3345 5432 654 3 cy5 2324 .95 6 NA 4345 432 100 4 cy3 2324 .95 7 2 3345 5432 654 4 cy3 2324 .9