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Hi brendaf
I have a dataset of ~140,000 ASVs that I want to tun through optimotu. The ASVs have been assigned taxonomy. Do you have any examples or recommendations for preparing the ASVs before run…
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Hi Chi,
This may be a naive question and tried a couple of things. We are trying to subset the microtable after querying the FungalTraits database to a specific lifestyle: "plant_pathogens". But …
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```
~/matlab/AstroPack$ ls -R | grep .asv
test_sphere_move.asv
test_sphere_offset.asv
test_spherical_triangle_circum_circle.asv
test_area_sphere_polygon.asv
test_sphere_move.asv
```
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Hi
I have data from multiple Illumina MiSeq runs, that I want to analyze together. Some of the runs were sequenced with a v2 kit (2x250 cyc), some with a v3 kit (2x300 cyc). All of the runs cover t…
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Hi DADA2 developer,
I have a high-level conceptual question - I've seen people clustering ASVs into OTUs at certain similarity threshold for various purposed. How is that different from directly us…
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There are now a bunch of front-end (CLI) tools which handle dependency resolution and installation of environments, including `rip`, `rye` (both already dropped out[^2]), and `uv`.
A key issue is …
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Hi, I'm trying to run spiec easi on a dataset with sequencing data.
I encounter this error message :
Error in if (max < min) stop("Did you flip min and max?") : argument is of length zero
I filte…
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It would be great to implement [ASV](https://github.com/airspeed-velocity/asv) or similar so we catch any changes that introduce a performance regression.
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Hi,
I have ASVs across a set of samples- can I run these through lotus 2 lambda using the taxOnly flag?
My guess is I need to create a fasta from the ASVs but then I wont have any quality score…