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During our May test run, collab filtering was successful in the search phase in our own container, but failed during the subsequent scoring step.
Logs from the test run show that it was failing a…
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I am processing 2x300 MiSeq 16s data using the 341F-806R primers. Initially I removed the sequencing primers using BBduk as ambiguous bases were causing issues with chimera checking (17 and 20 bp resp…
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I have been learning how to use the DADA2 software for my 16S/ITS analysis. One value I need to report is the average quality after trimming/filtering for the reads in each sample. I am following th…
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I would like to suggest an approach that I have found to dramatically improve the application of dada2 to fungal ITS amplicons. Some adapter removal programs use an alignment of the paired reads to tr…
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Better tagging and categorising for surfacing mixed resource types
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Hi everyone,
When I ues a 16s full-length sequence in qiime2 for analysis，there are some problems in step “Sequence quality control and feature table construction”. As shown below：
DADA2 R package …
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I'm using dada2 in an automated pipeline. Sometimes after demultiplexing and trimming primers, there are files with 0 reads.
When I have a file with 0 reads, the following rule fails:
```
libra…
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*Current Status:
[](http://fffff.at/speed-project/)
Very special thanks to @gustavnikolaj and everyone involved.
@sindresorhus @NARKOZ @roblarsen @Luit…
ghost updated
5 years ago
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Hello everyone,
I am very happy with dada2 results but I am scratching my head for a couple of hours thinking how I could filter uniques obtained with paired end reads merging (hence the resulting s…
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## Issue
Hey, I'm having an issue with the filter.
I am filtering ~6000 items, and when I click the upside down triangle that shows filter options, the page halts. I have many items with the…