-
Hello, I have two layers of data: the ATAC-seq results and the HTO hashtags. I encountered the following error when scaling the data, even if I skipped the demultiplexing steps:
```
library(Signac)…
-
Hi, I am running into a weird indexing error for some samples.
I run the following command with us.gcr.io/broad-dsde-methods/cellbender:20230403:
cellbender remove-background --input RawMatrix.h5a…
-
Hi, I deconvoluted my CARD object but it does not look like the original tissue at all. what could be causing this?
this is deconvoluted from CARD
but my original image looks like this:
…
-
https://mp.weixin.qq.com/s/V-t3uzaISVdkDt0P7Uig8w
-
Hi,
Thanks for this great package. I have a question about the simulation part of the paper. Regarding the simulation of a single-cell RNA sequencing (scRNA-seq) dataset, I'm considering the possibil…
-
https://mp.weixin.qq.com/s/_ggmd5FpVszU5fDTdFlwTQ
ixxmu updated
5 months ago
-
Hi @igrabski
Thank you for developing this elegant and useful tool!
I have a question related to the scRNA-seq gating plots that you show in your paper. Would you be willing to share the code to …
-
**User story**
As an SSR, I would like to be able to submit a collection of tubes containing banked PBMCs for cDNA prep, as part of the "scRNA Core" pipeline.
**Who are the primary contacts for t…
-
Hello,
I have a spatial dataset that is twice as large as the single cell. 60,000 vs 30,000.
Attempting to map the cells crashes for me, but considering that my single cell data has multiple pa…
-
How to obtain standardized data after batch effects removal by Harmony for differential analysis between two groups of cells?