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I'm running MacOS 10.15.7 on a MacBook Pro (16-inch, 2019), and compiling with Rust 1.52.0.
I first observed that in my `iced` application, in a scrollable, where the scrollable content has ~1000 l…
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Hi,
I was wondering if you could help me with the following urgent issue: our protein lane has 34k cells (after mapping with CITE-seq-Count using -cells option, no whitelist), RNA lane has 11k cell…
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Running TRUST4 on 3' 10X genomics RNA-seq results in B cell inference where no B cells are present, any advice on fixing this issue?
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Hello,
Sorry to bother you again.
I'm attempting to run Novel-X on the following 10x Genomics bam file : ftp://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/10XGenomics…
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Hi,
I have been meaning to try this tool for some time now. We do a lot of single-nuclei RNA-seq, as a result we end up capturing lot of reads from the pre-mrna . In order to deal with that , 1…
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Project: ENCODE
So - libraries for next-generation sequencing used to all be made by hand. However, we are starting to see the emergence of platforms (machines) which do the library construction i…
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Hello, could you provide with the processed and annotated scRNA-seq reference data, which is the "FlexSeuratV5.rds" in FlexSingleCell.R script? Thank you very much.
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Dear authors,
Please provide information needed to execute your pipeline:
1) what is the format of the samplesheet
2) how can one know barcodes before running any analysis?
3) What is BARCODE_STAR…
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Dear developers,
I wanted to use your tool enclone on 2 bcr files from 1 donor, before and after treatment.
However, I get following message:
```
Significant barcode reuse detected. If at le…
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@sjfleming Thanks for the great tool. I really appreciate for the amazing calculation.
When I used the tool for my 4 samples, I also met some problems.
**Q1: 3 different learning-rate for sampl…
GLLMU updated
3 weeks ago