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Hi Vadim,
I have run some label free single cell samples on my timsTOF HT using slicePASEF. I have different cell amounts per run, as you can see from the file names. When I try to run this, it see…
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Hi,
When running the denovo workflow we get the following error:
``
terminate called after throwing an instance of 'std::__cxx11::basic_string'
.command.sh: line 19: 21749 Aborted …
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### Operating System
macOS
### Other Linux
_No response_
### Workflow Version
v1.2.1
### Workflow Execution
EPI2ME Desktop (Local)
### Other workflow execution
_No response_
### EPI2ME Versi…
jh4yd updated
3 months ago
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The "c." and "p." HGVS notations are gene-based and can only be understood in reference to a specific gene isoform. For example, "SRF:c.25A>G" describes a variant of basepair 25 of the SRF gene (refe…
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Dear Vadim,
DIANN is really helpful for our proteomic research.
We recently met one problem. For example, in one batch of over 300 continuous sequenced data, 12 of them could not be processed in o…
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3 transcripts were identified in a sample. Their TPM values add up to ~546,000 and not 1 million like I would expect.
# stringtie -p 16 -j 10 -c 10 -s 10 -o output.stringtie.gtf input.Aligned.sorte…
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Hello,
I am currently following the `kallisto | bustools` tutorial on processing nuclear RNA-seq, which runs `bustools count` on spliced and unspliced transcripts. I know that you can specify `--g…
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I'm triggering `RuntimeError('There exist transcripts missing length information.')`
We are following the example: https://emase.readthedocs.org/en/latest/examples.html#estimating-allele-specific-exp…
travc updated
9 years ago
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Hi Heng,
I use the `-x ava-ont` option in minimap2 to get all-vs-all overlaps of ONT RNA-seq reads.
I also output the matching minimizers’ positions in each read for a matched read-pair.
In…