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Thanks for your work on this promising tool! In regards to its applications to metagenomics, can radEmu handle length normalized data as input? In metagenomics, it is common to normalize counts to the…
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I'm trying to run your code and I saw the requirement is BLASR 2.0 but on the BLASR github page installing legacy BLASR isn't possible. So I installed the lastest version (BLASR 5.1) but that version …
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Hi Benjamin,
Peggy here. Thank you for the help the other time for the filterAndTrim issues.
This time, also another trimming issues, but with ITS2 sequences. I followed your protocol for the I…
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![grafik](https://user-images.githubusercontent.com/19622117/119157433-79009580-ba55-11eb-863d-f80c51df7de6.png)
I think my error model is not converging. I included all the reads I possibly c…
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The goal of the guard rails is to provide users with informative warnings when their analysis could potentially be compromised. We want to create a system where we can have multiple guard rails for di…
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Hello,
I am relatively new to DNA sequencing analyses so I appreciate the detailed tutorials and feedback on previous issues.
I have 16s rRNA data from 3 different sequence runs using the same pri…
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Hi,
I am new to PacBio technology and I am currently working on 16S sequences generated from both Illumina MiSeq and PacBio Sequel II tech. I have 3 different projects including different plant tis…
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Our use case is variant calling on the output of a targeted amplicon assay on many samples in parallel. The way that `clair3_c_impl.sh` parallelizes the pileup calling step in this case is inefficient…
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Heya,
I have two runs (rep1, rep2) of 16S amplicon sequencing for 40 samples. The samples in 'rep1' are named like '1062-1-sampleNo1.R1.fastq' through '1062-1-sampleNo40.R1.fastq' and '1062-1-sample…
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#### Are you using the latest version of [samtools](https://github.com/samtools/samtools/releases/latest) and [HTSlib](https://github.com/samtools/htslib/releases/latest)? If not, please specify.
(ru…