-
Hi,
For `sci-Plex`, the cell barcode position is not a fixed value.
R1: { **9-10 bp** hairpin barcode }-{CAGAGC}-{ 8bp UMI }--{10 bp RT barcode}
How could I generate the `readTable` for this read…
-
At some point the default genomes file at [https://s3.amazonaws.com/igv.org.genomes/genomes.json](https://s3.amazonaws.com/igv.org.genomes/genomes.json) was updated such that chromosomeOrder became a …
-
When running `bindings.design_primers()` or `bindings.designPrimers()` from primer3-py v 2.0.1 the jupyter kernel crashes.
I tried with different kernels, Py 3.8 to 3.12, all crashed the same way.
…
-
I successfully installed the GMATA software on my MAC and was able to run it upto the Gbrowse viewing. While performing the marker designing I was stuck. The program started running and four output fi…
-
https://mp.weixin.qq.com/s/gqc9Q-Y-_LX4LptnWOT7qw
ixxmu updated
5 months ago
-
I have already removed the spurious pools of barcodes that weren't in the experiment.
Do we need/want all the extant columns? They are:
design | dialout | gene_id | gene_symbol | oligo | sgRNA | sou…
-
Dear,
I'm trying to use the Capture function in Oligo to design probes against the mouse genome mm10. I'm using the following code:
`python design.py Capture -f GRCm38.primary_assembly.genome.…
-
Hi,
Could you teach me why this error happen??
Traceback (most recent call last):
File "/lustre7/home/t-goto/oligo-0.1.1b/design.py", line 544, in
c.extract_repeats().calculate_density().…
-
First of all, thank you so much for creating this pipline and your efforts to improve and simplify probe design strategies.
I tested the pipeline and generated newBalance DNA probes for chromosomes…
-
We have generated some sequencing data from short (50 bp) oligonucleotides with strand-specific modifications and I was wondering if there is a way to use Remora to identify signatures of these modifi…