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I am trying to just learn how to use the tool and am having issues with the first step (species taxonomy) when running samples downloaded from NCBI. I ran the test script and went through the tutorial…
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Hello,
I am a doctoral student working on a microbiomes project and did an principal coordinates analysis in Phyloseq. While I recognize that PCA and PCoA are not exactly the same, I was wondering if…
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Post your lay and scientific abstracts below. Aim for a maximum of 250 words for both. Thanks!
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Leaving it vague for now. Big updates will happen soon.
joey
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Hello,
I am trying to bring in otu_table.biom, mapping file = SMP_Run1_Map.txt, and a tree rep_set.tre
``` S
otufile = "otu_table.biom"
mapfile = "SMP_run1_Map.txt"
treefile = "rep_set.tre"
run1
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> Version: 1.31.9 (21)
> iPhone 7 Plus Version: 10.3.2
> Wifi
**Issue: After unsubscribing from a bot after accessing bot via HS, this change doesn't reflect on the bot when accessing the same bo…
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snowball crashed:
**COMMAND LINE:**
snowball -f R1-trimmed.fq -s R2-trimmed.fq -m Pfam-A.hmm -i 225 -r 140 -p 15
**STDOUT**
This hmmsearch binary will be used:
"path to hmmsearch"
Using temporar…
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Mentioned in another issue [here](https://github.com/benjjneb/dada2/issues/41#issuecomment-236462931), it seems the best practice way to estimate the error rates on a subset of the data is to pick som…
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I am unsure if this is the most appropriate place to post this issue. I realize this may be more of a comment on the paper in question than DADA2, however I am specifically interested in the use of DA…
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I am currently working with two MiSeq runs worth of V4 vaginal sequencing and attempting to do the CST generations like you describe in your (fantastic) [CST Analysis: Vaginal Samples
](http://statwe…