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Hi,
Thank you for this nice package. I am analysis my scATACseq and matching scRNAseq data using ArchR. I would be interested to use your package on my ArchRproject but it is not implemented. Do you …
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Hi. I am new to the scRNA-seq analysis, and I have exploring your program to perform analysis.
How can I generate input data like the example you provided, Goolam?
I used Seurat to combine matrix.mt…
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https://mp.weixin.qq.com/s/ONPpTdaaoWz4CsWVajKBeg
ixxmu updated
2 years ago
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Hello,
I recently performed a 10x Genomics cell hashing experiment using 8 hashtags to label 2 treatments at 4 different timepoints. I'm wanting to compare treated vs. untreated cells at each tim…
ghost updated
2 years ago
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https://mp.weixin.qq.com/s/O5xL-QQXROSlWfkVeioqRw
ixxmu updated
2 years ago
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I have been using splatter to generate scRNA synthetic data. However, when I used the generated synthetic data for the Seurat object, I found some clusters do not have a single marker gene (or sometim…
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Hello,
Is it possible to combine bulk RNA data with single cells expression data for analysis in Seurat? any special guidelines?
Thanks!
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Hi, Greetings! When I tried to submit my 10x-scRNA-seq data file for analysis using the code below (with my bam file and barcodes.tsv, not shown here):
mgatk tenx -i ${outdir}/outs/possorted_bam.ba…
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https://mp.weixin.qq.com/s/ZCOoeQU6ysK9G8apTnfjvQ
ixxmu updated
2 years ago
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Hello,
More of a question rather than an issue. I was reading on `MiloR `and wanted to use this tool to identify neighborhoods in my scRNA-seq dataset that correlate in abundance with a treament of…