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Dear all,
We are analyzing some patients with this fantastic program and trying to learn the meaning of the results we obtain. On the graph we obtain a disperse point pattern (see image below):
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Hi, I ran the example command
tombo resquiggle path/to/fast5s/ genome.fasta --processes 4 --num-most-common-errors 5
and received the following error:
[15:45:46] Loading minimap2 reference.
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**User story**
As NPG we would like an extra "Contaminated Human Data Access Group" field on the New Study Page so that access to the "likely human" read data that NPG already creates separately.
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Hi Akdes,
I have been working my way through the pipeline and have run into an issue that I have been trying to work through the past few days with no luck.
I have no issue with all the preceding …
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Using `sc_exon_mapping` as an example: it would be convenient to be able to supply a `GRanges` object directly as `annofn=`, especially in situations where some editing of the annotation files are nec…
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Hello, I am interested in starting using TRUST4, and was wondering what is the best approach to run it on SMARTer data, in terms of parameters, pre-processing, etc.
I run a subset of 100K paired-en…
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**Describe the problem concisely**.
RNA-Seq provides quantitative measures of RNA abundance in the sequenced cell types. However, a major question of interest is typically quantitative: is the protei…
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- Open access data can be used by the QC WG, Mcgill has downlaoded this data and done some processing and think other folks can use this.
- We will stage this data in Object storage in Collab. We wa…
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Hello,
Recently we have been using hybpiper to assemble thousands of BUSCO single-copy genes from metagenomic reads (2x150bp). The target file is amino acids, and we set the coverage cutoff for spa…
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Creating this thread to post potentially useful info about _Pichia pastoris_/_Komagataella phaffii_ that people come across.
Quick background: _Pichia pastoris_ (now named _Komagataella phaffii_) i…