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For example, one can imagine using Python to carry out some transformations on flow cytometry data, but then use other flow cytometry tools to visualize and further process the data. Since all these …
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Flow cytometry fcs files have a long text header before the binary portion starts (https://en.wikipedia.org/wiki/Flow_Cytometry_Standard), in particular an example there has the binary data starting a…
pcm32 updated
3 years ago
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Dear @CUHIMSR ,
Thank you for sharing this important tool for batch effect correction. I had a question regarding the reference sample named "anchor", i downloaded the fcs files from the vaccinati…
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Hi gitdemont,
are you planning to add support for the flow imaging files generated by the machine FACSDiscover S8? The imaging files are saved in a .cvw file, but images can be exported as .TIFF.
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Dear Jhuang
Thanks for the great package that you have developed for the flow cytometry analysis. I noticed that once we import the fcs files and make a flowspy object, the scaled values are importe…
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Hi,
I have encountered a problem when using cytofkit (via GUI) and would like to know if anyone else has encountered something similar. cytofkit is not outputting any .fcs files in the result direc…
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From Nathan:
Hi Ben, David, and Heather,
My name is Nate and I work with Lili at NIST in the flow cytometry consortium. I'm trying to download all the FCS files from WG2 (~2600 files) onto an analys…
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Hi,
I hope you can help me with this. I am trying to analyze flow cytometry data acquired in a Cytek Aurora. I have 230 files, but I am using only 12 files from 4 different batches to see if I can …
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Hi Everyone,
I've been kicking this idea around for a little bit. Our group does a lot of batch processing of input files when we run our pipeline for flow cytometry data. Sometimes the output of a…