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### Description of the bug
When running nf-core/viralrecon, the medaka minion throws an error. Seems the model cannot be downloaded via the medaka minion script. scm file with github credentials is p…
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Hi, i am currently trying to trim a fastq file i got from publicly available datasets with Nanofilt.
I have the following codes:
[nanofilt_script.txt](https://github.com/user-attachments/files/160…
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### Description of the bug
It is my first time running nf-core/viralrecon pipeline using a sequence from nanopore (Minion). I used the following script but th Pipeline completed with errors. Is the…
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Hi there,
I am trying to basecall pod5 files using guppy and it seems like it does not recognise the pod5 files. I am using version 6.1.7
Here is my script:
guppy_basecaller --input_path /mn…
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Hi! I´m trying tu run Masurca with PacBio and Nanopore reads. I read that it is possible to supply them all as NANOPORE type. How do you add more than one file of Nanopore reads? I tried this:
NANO…
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I am attempting to use the prokseq-v2.0 pipeline to evaluate some data obtained by directly sequencing RNA using an nanopore device ( ONT MinION). I can test-run the pipeline using the provided data, …
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Hi,
I have Nanopore sequencing data for the 16s gene of microbioal communities. Can dada2 be used for this type of sequencing data, and if not, is it in development? Thanks!
Best wishes,
Carla …
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Hi there
Loving this --revcomp function!
I was wondering if it is possible to be selective in which of the reads to keep, i.e., reads identified with adapters in the 5'-3' or reads identified as…
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Hello Aleksey,
I was wondering if I could use Nanopore and Pacbio together for the hybrid assembly. We have about 70x pacbio, 20x long-read Nanopore, and ~ 100x Illumina for a 650 Mb genome. What …
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Hi,
We bought the ONT nanopore sequencing machine MinION, and tested using fix-length library (e.g. Eco.li). A confusing result was observed during the sequencing, we found a significant drop of ac…