-
Hi! Thanks for developing such a comprehensive tool for sgRNA design. I am wondering if it is possible to add on-target scores for SaCas9 using models like Azimuth 2.0 or something else. It will be ve…
-
Hi,
I am trying to design sgRNA for campylobacter, but realised the campylobacter genome sequence is not available in the shiny app. Is there a way to upload my own genome sequence into the app? …
-
This is urgent. I just tried meta-mode design guideRNA for human EMX1 and EGFR, but has error retrieving sgRNA sequence? Also there's no longer a clear / refresh button, is this removed intentionally?…
-
### Description of the bug
The process NFCORE_CRISPRSEQ:CRISPRSEQ_SCREENING:MAGECK_FLUTEMLE starts the FluteMLE command using the data of the gene_summary.txt file. It downloads several pathway files…
-
For some ENST numbers and HGNC we run into the error `AttributeError: 'str' object has no attribute 'iteritems'` (whole error at the bottom). This happens because sometimes the `edit_map` becomes an e…
-
**Analysis of tested guide RNA’s used for gene editing via CRISPR to predict optimal future guide sequences**
Despite the apparent simplicity of this sequence matching strategy to target the CRISPR…
-
I am trying to run getCrispraiScores using the vignette example, but run into the following error.
***Docker install:***
```
sudo docker run --rm -ti -p 8788:8787 -v $(pwd):/guide_design -e DISAB…
-
[config_mApple2_gRNA_100919.txt](https://github.com/zhangchonglab/CRISPRi-functional-genomics-in-prokaryotes/files/3714579/config_mApple2_gRNA_100919.txt)
first of all, nice tool!. I have been tryi…
-
### Error: mageck mle; how to solve this problem?
> mageck mle --count-table BatchCorrect.txt \
> --design-matrix designmatrix.txt \
> --norm-method control \
> --control-sgrna ctrl_sgrna_list…
-
Dear brocklab research group:
You did an excellent job in the field of lineage-tracing. Our research group is using the technology you developed to construct the ClonMapper library.In or…