I used your code for paired-end data and calculated FPKM of genes. However, if my count file say had 5 genes and 30 samples, the FPKM was returned for only 2 genes, 30 samples. For all the genes, there were a few samples with 0 counts. So I am not able to understand why FPKM for certain genes were not computed.
Hi
I used your code for paired-end data and calculated FPKM of genes. However, if my count file say had 5 genes and 30 samples, the FPKM was returned for only 2 genes, 30 samples. For all the genes, there were a few samples with 0 counts. So I am not able to understand why FPKM for certain genes were not computed.
Thanks!