For families of reads where the consensus base does not match our reference, we should check to see what fraction of the reads supported that incorrect base. I expect to see errors scattered throughout the reads, but only piling up in PCR or other chemistry-related events.
This will also get us a clearer/better idea as to how effective BMFTools is at eliminating sequencing errors by separating chemical from optical errors.
For families of reads where the consensus base does not match our reference, we should check to see what fraction of the reads supported that incorrect base. I expect to see errors scattered throughout the reads, but only piling up in PCR or other chemistry-related events.
This will also get us a clearer/better idea as to how effective BMFTools is at eliminating sequencing errors by separating chemical from optical errors.