AlejandraArnedo
commented
1 year ago Hi Yun,
I couldn't open the images with the links provided.
Alejandra
From: Yun Zhang @.> Sent: Wednesday, July 19, 2023 16:06 To: AlejandraArnedo/Wound-healing-size-tool @.> Cc: Subscribed @.***> Subject: [AlejandraArnedo/Wound-healing-size-tool] Issues with the tool for extreme cases (Issue #9)
Greetings, I've been using this wound healing size tool since it first came out and lately I have been noticing some issues with it when it comes to analyze images with extreme cases.
Scenario#1
Image link: https://www.dropbox.com/s/2wiacvwf3zwpblo/Case%231.tif?dl=0
Scenario#2
Image link: https://www.dropbox.com/s/jgawnkieuv8henl/Case%232.tif?dl=0
The scale I used for both images are: Distance in pixels:1, Known distance: 0.43, Unit: um.
Thank you for your help in advance and I look forward to hearing back from you.
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yunzhang77
Greetings, I've been using this wound healing size tool since it first came out and lately I have been noticing some issues with it when it comes to analyze images with extreme cases.
Scenario#1 Image link: https://www.dropbox.com/s/2wiacvwf3zwpblo/Case%231.tif?dl=0 This image represents a scenario where all cells are dead due a treatment and the scratch has, in a way, 100% percent area and a width that is same to the length of the image. However, which the tool is measuring it, it reports a good enough percent area (98.230%), but the width is way off (2.602 um).
Scenario#2 Image link: https://www.dropbox.com/s/jgawnkieuv8henl/Case%232.tif?dl=0 This image represents a different scenario where the scratch is separated by cells. The tool detects the opening at the bottom as the scratch with a 17% area and 18um width. While the percent area may be fine, I also think the width measurement is off for this case. I am also wondering if there is a why where I can define multiple ROIs as scratches and do the corresponding measurements.
The scale I used for both images are: Distance in pixels:1, Known distance: 0.43, Unit: um.
Thank you for your help in advance and I look forward to hearing back from you.